甲氧氯普胺对吗啡依赖性小鼠戒断症状和脑区cGMP含量的影响  

EFFECTS OF METOCLOPRAMIDE ON DRUG WITHDRAWAL SYNDROMES AND BRAIN cGMP LEVEL IN MORPHINE-DEPENDENT MICE

作　　者：陈一燕[1] 陈崇宏[2] 

机构地区：[1]厦门市第一医院药剂科,厦门361003 [2]福建医科大学药理教研室,福州350004

出　　处：《中国药物依赖性杂志》2004年第3期191-193,共3页Chinese Journal of Drug Dependence

摘　　要：目的 :观察甲氧氯普胺脑室给药对吗啡依赖小鼠戒断症状的影响以及腹腔给药对小鼠不同脑区cGMP含量的影响。方法 :皮下注射 (sc)盐酸吗啡建立吗啡依赖小鼠实验模型 ,在纳洛酮催促戒断前 30min侧脑室微量注射甲氧氯普胺 ,观察其急性给药对吗啡依赖性戒断症状的影响 ;用放射性免疫法观察腹腔注射 (ip)甲氧氯普胺对吗啡依赖小鼠小脑、大脑皮层、海马及丘脑四个脑区cGMP含量的影响。结果 :甲氧氯普胺 (1 0mg·kg- 1 )可有效抑制纳洛酮催促的吗啡依赖小鼠的跳跃反应 (P <0 0 1) ;吗啡依赖小鼠四个脑区中cGMP含量均低于正常鼠 (P <0 0 1) ,甲氧氯普胺急性给药 (2 0mg·kg- 1 ,ip)可使吗啡依赖小鼠cGMP接近正常水平。结论 :中枢神经系统是甲氧氯普胺抑制吗啡依赖小鼠戒断跳跃反应的主要作用部位 ;Objective: To study the effects of metoclopramide (Meto) on drug withdrawal syndromes and brain cGMP in morphine-dependent mice. Methods: Mice were administered(sc) with morphine hydrochloride to establish morphine dependence model. Withdrawal syndromes were precipitated by naloxone(ip). cGMP concentrations of cerebellum, cerebral cortex, hippocampus and thalamus were determined by radioimmunoassay. Results: Meto(1 mg·kg -1 , icv)inhibited the jumping of morphine-dependent mice effectively (P<0 01); concentrations of cGMP of four brain areas were lower than those of normal mice (P<0 01); Meto (20 mg·kg -1 , ip) increased the cGMP of the four brain areas to normal level. Conclusion: Inhibitory effects of Meto on drug withdrawal syndromes in morphine-dependent mice work through central nervous system and the change of brain cGMP level might be one of the mechanisms of the inhibition of Meto on drug withdrawal syndromes in morphine-dependent mice.

关 键 词：甲氧氯普胺 吗啡依赖 小鼠 戒断症状 脑区cGMP 含量检测 

分 类 号：R595.5[医药卫生—内科学] R971[医药卫生—临床医学]