人类中脑神经祖细胞的体外扩增及诱导分化  

The proliferation and differentiation of human mesencephalic progenitor cells in vitro

作　　者：刘爽[1] 田增民[1] 王亚奇[1] 周英[1] 于雪[1] 林鸿[1] 

机构地区：[1]海军总医院神经外科,北京100037

出　　处：《军医进修学院学报》2004年第6期401-403,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家"8 63"计划项目 ( 970 5 18)

摘　　要：目的 :探讨人类中脑祖细胞体外分离、培养及向多巴胺能神经元诱导分化的方法。方法 :利用neuro sphere法 ,在低氧分压的情况下建立中脑祖细胞克隆 ,并用纹状体培养上清对其进行诱导分化 ,观察TH阳性神经元的分化比率及成熟度。结果 :中脑来源的神经祖细胞克隆 ,能以neurosphere形式生长 ,低氧分压对其克隆生长具有促进作用 ;纹状体培养上清可增加TH阳性神经元的分化比率 ,并能使TH阳性细胞具有更成熟的多巴胺能神经元的形态特征。结论 :低氧分压更适于中脑神经祖细胞的体外扩增 。Objective:To study of the method about dissection, proliferation and differentiation of mesencephalic progenitor cells in vitro. Methods:Neurosphere method was used to expand the mesencephalic progenitor cell clones in vitro in 3% atmospheric oxygen. Striatal culture conditioned media was used to induce the mesencephalic progenitor cells differentiated into mature DA neurons.Results:The mesencephalic progenitor cells were successfully expanded in vitro using the epidermal growth factor (EGF) and fibroblast growth factor-2(FGF-2) in 3% atmospheric oxygen. Following incubation in differentiation media containing striatal culture conditioned media, up to 2% of the precursor cells converted into TH-immunoreactive (TH-ir) cells, which exhibited mature morphological and functional properties of dopamine neurons in culture. Conclusions:Dividing human mesencephalic precursor cells can be isolated and expanded in vitro in the presence of reduced atmospheric oxygen, and striatal tissue induces some of these cells into TH-ircells.

关 键 词：中脑 神经祖细胞 体外扩增 诱导分化 多巴胺能 神经元 

分 类 号：R338[医药卫生—人体生理学]