抗肿瘤坏死因子单克隆抗体对心肌细胞凋亡的影响  

The effect of anti-TNF-mAb on the apoptosis of myocardial cells

作　　者：刘亮[1] 张万义[2] 叶平[1] 

机构地区：[1]解放军总医院老年心内科,北京100853 [2]解放军总医院军医进修学院临床医学博士后流动站,北京100853

出　　处：《军医进修学院学报》2004年第6期404-405,共2页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的 :探讨抗肿瘤坏死因子单克隆抗体对持续缺血和缺血再灌注损伤心肌细胞凋亡的影响。方法 :大白鼠随机分为七组。以活结结扎左冠状动脉的前降支 ,分别造成阻断冠脉血流和再灌注。以缺口末端标记法 (Tunel法 )标记凋亡细胞。结果 :凋亡细胞原位标记与半定量分析表明 :抗肿瘤坏死因子单克隆抗体处理组与持续缺血组、缺血再灌注组比较心肌细胞凋亡程度明显降低。结论 :缺血再灌注损伤和持续缺血可导致心肌细胞凋亡 ;Objective:To study the effect of Anti-TNF-mAb on the apoptosis of myocardial cells during the continuous ischemia and the ischemia reperfusion injury. Methods:Wistar rats were randomly divided into 7 groups. Ligate the anterior descending branch of the left coronary artery with slipknot to block the blood flow and reperfuse the myocardium. The apoptotic cell was labeled by TUNEL. Results:In situ apoptotic cell labeling and semiquantitative analysis reveal: The degree of the apoptosis of myocardial cells decreased significantly in the Anti-TNF- mAb-administrated groups compared with continuous ischemia group and ischemia reperfusion injury group. Conclusion:Continuous ischemia and ischemia reperfusion injury can result in the apoptosis of myocardial cells; the preconditioning with Anti-TNF- mAb can lessen the degree of the apoptosis of myocardial cells signigicantly.

关 键 词：抗肿瘤坏死因子 单克隆抗体 心肌细胞 细胞凋亡 缺血再灌注损伤 

分 类 号：R542.2[医药卫生—心血管疾病]