应用自体软骨细胞移植物修复关节软骨缺损  

Experimental study of repair of articular cartilage full -thickness defect by tissue engineered autograft

作　　者：池光范[1] 侯宜[1] 侯立中[1] 颜炜群[1] 杨同书[1] 

机构地区：[1]吉林大学再生医学科学研究所,长春130021

出　　处：《中国地方病学杂志》2004年第6期516-519,共4页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(39870781;30271319)

摘　　要：目的 通过体外扩增关节软骨细胞,构建软骨组织工程移植物,观察移植物对自体关节软骨损伤的修复作用。方法由8周龄成年兔髌骨外侧缘获取关节软骨,分离软骨细胞,体外扩增后,与牛Ⅰ型胶原、人血冻干纤维蛋白原、凝血酶按一定比例混合,制成软骨培养物,体外培养4 d。用于对成兔自体关节软骨损伤的移植修复。结果 成年家兔关节软骨细胞体外单层培养至第2代时开始出现去分化现象,可利用生长因子抑制去分化;该移植物的移植修复效果良好,修复后12周移植物与周边正常组织结合紧密、齐高,细胞趋于柱状排列,已形成透明软骨组织。结论 利用体外扩增的方法能获得足够量表型良好的关节软骨细胞,当应用胶原一纤维蛋白为载体制备出软骨组织工程培养物后,能较好地修复自体关节软骨损伤。Objective To investigate a new method of repair of full-thickness articular cartilage defect by tissue engineering autograft. Methods Chondrocyte were isolated from articular cartilage of adult rabbit patella in lateral border, digested by collagenase Ⅱ, and expanded in vitro by monolayer culture to passage 3. The cells were harvested and mixed with bovine type I of collagen, blood freeze-dry fibrinogen and thrombosin according to a certain proportion to culture in vitro for 4 days and then was autografted into the defect of the rabbit in femur near knee joint and under pateEa. Results The cells of adult rabbit was appeared dedifferentiation in in vitro monlayer culture at passage 2; the growth factor of bFGF and TGF -b 1 can usefully overcome this problem; the articular defect repair showed good result's. The defect filled with hyaline-like cartilage, characterized by its smooth surface and well-fusion with is surrounding tissues, and the high degree same to sorroundiing normal cartilage, the chondrocyte in repaired tissue arranged in column after grafting after 12 weeks. Conclusions These methods could culture adequate cells in vitro monolayer culture and using collagen and human fibrin as supporter have good repair effect in large full-thickness repair models.

关 键 词：自体移植物 关节软骨缺损 软骨细胞 胶原 纤维蛋白 

分 类 号：R687.3[医药卫生—骨科学]