慢性氟中毒大鼠肝脏损伤机制及抗氧化中药的拮抗作用  

Study on the lesion of the liver of the rats with chronic fluorosis and the therapeutical effect of the Danshen-gypenosides composite

作　　者：王守立[1] 高勤[1] 于燕妮[1] 刘家骝[1] 梁任又[2] 徐云华[2] 金嗣昭[2] 

机构地区：[1]贵阳医学院病理教研室,550004 [2]中国科技大学结构与分析教研室

出　　处：《中国地方病学杂志》2004年第6期546-548,共3页Chinese Jouranl of Endemiology

基　　金：贵州省科委科研基金资助项目(B97-9)

摘　　要：目的 研究实验性氟中毒时肝脏自由基代谢及超微结构的变化,探讨氟中毒时自由基在肝脏损伤机制中的作用。方法 用Wistar大鼠复制出慢性氟中毒模型,每日腹腔注射丹参-绞股蓝中药复方制剂,6个月后处死。用电子自旋共振法及其他常规方法检测肝组织自由基代谢水平,用体视形态学分析肝组织超微结构的变化。结果 染氟大鼠自由基、丙二醛增高;SOD及GSH-Px活性降低;电镜观察线粒体肿大、体积密度和表面积密度增加;而抗氧化治疗组各项指标与正常组无明显差异。Objective To investigate the role of free radicals in the pathogenesis of the fluoride intoxication, and the ultrastructural damage in the liver of the rats with experimental fluorosis. Methods Wistar rats were fed with NaF to produce the animal model of fluorosis. Free radical content was analysed using electron spin resonance and the changes of the ultrastructure of the liver was analysed using morphometric method. Results In the fluoride exposed rats, a higher level of free radicals and MDA was noticed, the activities of SOD and GSH-Px were lower than that of control, while swelling, and increased volume density and surface density of the mitochondria in the liver cells was observed. Conclusions Excessive fluorine may induce biomembrane injury of the liver cells by inducing lipidperoxidation.

关 键 词：慢性氟中毒 大鼠 肝脏损伤 脂质过氧化 丹参-绞股蓝 中药制剂 

分 类 号：R595.2[医药卫生—内科学] R285[医药卫生—临床医学]