喂饲补肾中药大鼠的血清对成骨细胞的生物学作用  

Biological effect of serum of kidney-tonifying traditional Chinese drug-fed rats on cultured osteoblasts

作　　者：唐井钢[1] 李娟[1] 吴贺勇[1] 

机构地区：[1]南方医科大学南方医院中医科,广东广州510515

出　　处：《第一军医大学学报》2004年第11期1248-1250,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(30371840)~~

摘　　要：目的探讨喂饲补肾中药大鼠的血清对体外培养的成骨细胞的生物学作用。方法在新生SD大鼠头颅骨第2代成骨细胞培养液中加入不同浓度(低、中、高)的中药血清,并与空白组对照,分别观察成骨细胞的增殖、分化和矿化功能。结果补肾中药血清具有刺激成骨细胞增殖的作用,可提高碱性磷酸酶活性并增加矿化结节形成的数量。结论补肾中药血清具有刺激体外培养的成骨细胞增殖及分化成熟的功能。Objective To investigate the biological effects of the serum of rats fed with kidney-tonifying traditional Chinese drugs on cultured osteoblasts in vitro. Methods The culture media containing the serum from rats fed with the drugs at different doses (high, mediate and low doses) were used to treat the second passage of the osteoblasts from the skull of newborn SD rats, and the cell proliferation, differentiation and mineralization were observed. Results The serum stimulated the cell proliferation, enhanced alkaline phosphatase activity and increased the number of mineralized nodules in the cultured osteoblasts. Conclusion Kidney-tonifying traditional Chinese drugs can stimulate the proliferation, differentiation and maturation of cultured osteoblasts in vitro.

关 键 词：大鼠 成骨细胞 血清药理学 骨质疏松症 骨灵丸 中药制剂 

分 类 号：R285[医药卫生—中药学]