辅助肾小管装置免疫调节机理研究  

Immune modulation mechanism of renal tubule assist device

作　　者：严泉剑[1] 陈香美[1] 吴镝[1] 谢院生[1] 付博[1] 张利[1] 李志辉[1] 张宇梅[1] 马志芳[1] 卢英杰[1] 申力军[1] 刘秀娟[1] 孙雪峰[1] 

机构地区：[1]解放军总医院肾科

出　　处：《中国血液净化》2004年第11期605-608,共4页Chinese Journal of Blood Purification

基　　金：国家自然科学基金创新研究群体基金 (30 12 10 0 5 );国家 973基金(No .2 0 0 0 0 5 70 0 0 );第35批博士后科学基金 (2 0 0 4 0 35 0 5 4 )支持

摘　　要：目的　研究辅助肾小管装置参与全身免疫调节的机理。方法　分别用感染患者持续血液滤过(continuousvenovenoushemofiltration ,CVVH)超滤液和脂多糖 (LPS)干预培养人肾小管上皮细胞系HKC细胞 ,用RT -PCR、ELISA检测IL - 10转录水平和蛋白水平表达变化 ,用”transwell”方法检测IL - 10在HKC细胞内的转位特点。结果　RT -PCR、ELISA结果显示 ,HKC细胞暴露于感染患者CVVH超滤液或含LPS培养液后 ,IL - 10转录水平升高 ,上清中IL - 10蛋白水平升高。”transwell”方法检测发现IL - 10向两侧分泌。结论　辅助肾小管装置中的HKC可以通过表达IL - 10 ,参与全身免疫调节。Objective To study the immune modulation mechanism of renal tubule assist device (RAD) in end-stage renal disease (ESRD) therapy. Methods Cultured with serum of sepsis or medium contain lipopolysaccharide(LPS) on human kidney tuble epithelial cell lines HKC,the transcription and translation expression of interleukin-10(IL-10) were detected with RT-PCR、ELISA. The translocation of IL-10 in HKC stimulated with LPS was detected with “transwell”. Results The expression of IL-10 showed higher in HKC from renal tubule assist device stimulated with LPS than that in normal culture medium or ultrafiltrate. The HKC can secret IL-10 toward both apical and basal. Conclusion The HKC cells in RAD take part in immune regulation with the expression of IL-10 stimulated by LPS.

关 键 词：辅助肾小管装置 IL-10免疫 

分 类 号：R446.6[医药卫生—诊断学]