DHEA抑制大鼠转移性Morris肝癌的实验研究  

Experimental study on inhibitory effects of DHEAon transplanted Morris hepatomas in rats

作　　者：姜艳芳[1] 谭岩[1] 赵平伟[2] 刘力华[1] 方艳秋[1] 段秀梅[1] 松崎静司[3] 

机构地区：[1]吉林大学第一医院中心研究室,吉林长春130021 [2]吉林大学第四医院肝胆外科,吉林长春130011 [3]日本筑波大学胃肠肝病科

出　　处：《吉林大学学报（医学版）》2004年第6期860-864,共5页Journal of Jilin University：Medicine Edition

基　　金：国家留学基金委资助课题 (2 182 2 0 6 7) ;吉林大学临床医疗新技术新疗法基金资助课题 (2 0 0 10 3) ;吉林省卫生厅资助课题 (0 2 0

摘　　要：目的 :探讨去氢表雄酮 (DHEA)的体内抗癌作用及其机制。方法 :Buffalo大鼠 2 1只随机分为空白对照组 (n=5 ) ;肿瘤对照组 (把 Morris肝细胞瘤移植到大鼠的两肋皮下 ,建立 Morris肝细胞瘤移植的 Buffalo大鼠的体内模型 ) ,喂养基础饲料 (n=6 )、DHEA肿瘤组 (建立 Morris肝细胞瘤移植模型后 ,在喂养的基础饲料中添加 DHEA) (n=6 )和去氢表雄酮硫酸酯 (DHEA- s)肿瘤组 (建立 Morris肝细胞瘤移植模型后 ,在喂养的基础饲料中添加 DHEA - s) (n=4 )。分别喂养 4周后 ,应用流式细胞术检测大鼠的免疫功能 ,应用磁场型高分解能质量分析仪 (GC/ MS)方法测定大鼠体内类固醇的含量 ,采用免疫组化方法染色检测磷酸化的磷酸酶和张力蛋白同源物基因 (PTEN )蛋白表达 ,同时测定其对大鼠的毒副作用。结果 :DHEA肿瘤组的瘤重量 [(8.31± 0 .6 1) g]较肿瘤对照组 [(14 .5 7± 0 .5 6 ) g]显著减小 ,抑制率达到 4 3%。免疫组织化学染色显示 DHEA肿瘤组的磷酸化PTEN蛋白表达量增加 ,同时提高了 CD3、CD4阳性细胞百分率及 CD4 + / CD8+比值 ,与肿瘤对照组相比差异有显著性 (P<0 .0 5 )。 DHEA肿瘤组的胆固醇、甘油三脂及高密度脂蛋白的含量与空白对照组相比差异无显著性 ,未见其有毒副作用。结论 :DHEA对肿瘤具有明显的抑制作?Objective To investigate the inhibitory effects of dehydroepaimdrosterone (DHEA) on the growth of transplanted Morris hepatomas(7288CTC) in vivo in rats and its mechanism. Methods 21 Buffalo rats were randomly devided into 4 groups, including one blank control (n=5), one group for tumor-bearing control (n=6), and 2 experimental groups with DHEA (n=6) or DHEA-s (n=4). DHEA or DHEA-s was fed to the rats for 4 weeks immediately after Morris hepatomas (7288CTC) was implanted in both flanks. Phenotypes of the spleen lymphocytes were examined by flow cytometry, PTEN expression in tumor cells was detected with specific MoAb in immunohistochemistry. The steroid contents in rat sera were determined by GC-MS. Results Tumor weight of DHEA treated group was less than those of controls (P<0.05), the inhibitory rate was 43%. The positive rate of PTEN protein in DHEA tumor group was higher than those in the controls. The percentage of CD3 + and CD4 + T cells in the spleens of DHEA-feeding rats increased significantly compared with the control group (P<0.05). There was not significant difference of serum steroid,TG and HDL levels between DHEA treated group and the control group.Conclusion The DHEA can inhibit tumor by increasing the PTEN protein expression and immune function.

关 键 词：去氢表雄酮 肝肿瘤 基因 肿瘤抑制 磷酸酶和张力蛋白同源物基因 

分 类 号：R979.1[医药卫生—药品] R-332[医药卫生—药学]