一种测定小鼠长时间自发活动时空特点的新方法  

A novel method for continuously assessing the spatio temporal properties of locomotor activity in mice

作　　者：张琦[1] 魏尔清[1] 朱朝阳[1] 张世红[1] 张纬萍[1] 俞月萍[2] 陈忠[1] 

机构地区：[1]浙江大学医学院药理学教研室,浙江杭州310031 [2]浙江医学高等专科学校药理学教研室,浙江杭州310053

出　　处：《浙江大学学报（医学版）》2004年第6期509-514,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金 (30 2 714 98);浙江省科技厅科研基金 (2 0 0 2 C330 5 6 )资助项目

摘　　要：目的 :应用计算机视频跟踪技术 ,建立一种测定小鼠长时间开场自发活动的新方法。方法 :应用连续记录 2 2 h内小鼠在开场的自发活动 ,并用我们研制的 Analy Power1.1系统分析活动轨迹 ;以自发活动总路程、各区域活动路程及其占总路程的比例、各区域停留时间及其占总时间比例等指标 ,评价小鼠自发活动的特点。结果 :小鼠在清醒自由状态下 ,2 2 h内的活动表现显著的区域、时间依赖性特点。停留及活动主要集中在生活区 (食物和饮水区 ) ,其次周边区 ,中央区很少。小鼠在进入开场的第 1h内有明显频繁的活动 ,1h后活动明显减少。在活动趋于稳定后 ,小鼠表现出较明显的昼夜活动特点 ,夜间活动增加。结论 :本研究建立的方法 ,可客观、定量、连续测定小鼠自发活动时间、空间规律。Objective: To develop a novel method for continuously assessing the spatio temporal properties of locomotor activity of mice in an open field using a video tracking system. Methods: The locomotor tracks in the open field were recorded by video camera within 22 h,and analyzed by AnalyPower1.1 system that we developed recently.Total distance,distances traveled in different zones and their ratios to total distance; total time,times spent in different zones and their ratios to total time were used as indicators to assess the properties of locomotor activity. Results: In free and wakeful state,the locomotor activity of mice presented obvious regional and temporal properties.Mice preferred to stay in home base (food and water zones),and frequently visited the peripheral zones but seldom the center zones within 22 h.On the other hand,mice were most active within the first 1 h,and then their activity obviously decreased.After their activity became stable,the mice showed the obvious circadian variation of the activity as they were more active in the night. Conclusion: The novel method we developed in this study can continuously assess the spatio temporal properties of locomotor activity quantitatively and objectively. [

关 键 词：图像处理 计算机辅助 昼夜节律 行为 自发活动 开场 生活区 小鼠 

分 类 号：Q95-3[生物学—动物学]