低切应力对动脉粥样硬化形成及血管平滑肌细胞α-肌动蛋白和c-Myc蛋白表达的影响  

EFFECTS OF LOW SHEAR STRESS ON DEVEIOPMENT AND EXPRESSION OF α-ACTIN AND c-Myc OF VASCULAR SMOOTH MUSCLE CELLS OF COMMON CAROTID ATHEROSCLEROSIS

作　　者：王溪涛[1] 张炎[1] 刘波[2] 李玉泉 姜宗来[2] 

机构地区：[1]上海第二军医大学解剖学教研室,上海200433 [2]上海交通大学医学院力学生物学与医学工程实验室,上海200030

出　　处：《解剖学报》2004年第6期617-621,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (10 13 2 0 2 0 )

摘　　要：目的　为探讨低切应力对颈总动脉粥样硬化形成及其血管平滑肌细胞增殖的影响 ,建立了低切应力颈总动脉粥样硬化模型 ;并观察了血管平滑肌细胞的α 肌动蛋白和c Myc蛋白表达的变化。　方法　手术结扎家兔左颈外动脉 ,术后高脂饲料喂养。组织学和免疫组织化学方法观察粥样斑块的形成和α 肌动蛋白及c Myc蛋白表达的变化。　结果　在低切应力颈总动脉粥样硬化模型中 ,颈总动脉较早出现明显粥样斑块 ,管壁增厚 ,管腔内径变小 ;颈总动脉血管平滑肌细胞 (VSMC)的α actin含量减少、c Myc表达增高。　结论　低切应力促进了动脉粥样硬化斑块的形成 ,促使粥样硬化动脉VSMC增殖能力增强。Objective To investigate effects of low shear stress on atherosclerosis,the development of atherosclerosis and expression of αactin and cMyc proteins were studied in VSMC of common carotid artery under low shear stress. Methods We established the atherosclerotic model of left common carotid artery that presented low shear stress by the operation of ligating the left external carotid artery in rabbits.The histomorphology of the carotid arteries was examined by light microscopy.The expression of αactin and cMyc was studied by immunohistochemistry. Results The typical atherosclerosis was observed and the atherosclerotic lesion was obviously accelerated under low shear stress.The expression of αactin was downregulated and the phenotype of VSMC converted from contractile phenotype into synthetic phenotype,and expression of cMyc increased in VSMC of the artery under low shear stress.Conclusion This new model of atherosclerosis under low shear stress was successfully established,which provides a new tool for studying the effects of low shear stress on atherosclerosis.The results show that the atherosclerotic lesion is obviously accelerated and VSMC tends to proliferate in response to low shear stress.

关 键 词：动脉粥样硬化 切应力 颈总动脉 血管平滑肌细胞 Α-肌动蛋白 C-MYC蛋白 免疫组织化学 兔 

分 类 号：R543.502[医药卫生—心血管疾病]