汞、镉、铅联合染毒对小鼠DNA损伤的研究  

Study on Damage of DNA in Mice Induced by Mercury Cadmium and/or Lead

作　　者：胡晓磐[1] 周建华[1] 时夕金[1] 严丽萍[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2004年第5期595-597,共3页Suzhou University Journal of Medical Science

基　　金：江苏省自然科学基金社会发展项目(BS2001050)

摘　　要：目的 探讨汞、镉、铅对小鼠外周血淋巴细胞DNA损伤的联合作用及其机制。方法 连续2 d给昆明种小鼠腹腔注射不同组合的汞、镉、铅,末次染毒1 h后取血用单细胞凝胶电泳(SCGE)和3H-TdR掺入法检测DNA的损伤。结果 SCGE试验时,汞-镉-铅组DNA迁移距离明显高于其相应单独作用组,汞-镉组、镉-铅组分别与镉组比较,差异也有显著性;用3H-TdR掺入法同时进行实验,汞-镉组及汞-铅组与其单独染毒组比较,DPM值明显下降,汞-镉-铅三者联合染毒时,DPM值下降更为明显,说明DNA损伤愈加严重。结论 汞、镉、铅具联合毒性作用,三者联合染毒对小鼠外周血淋巴细胞DNA损伤更为显著。Objective To explore the joint injury actions of mercury, cadmium and/or lead on DNA in peripheral blood lymphocytes of mice. Methods The blood specimens were obtained from mice at the 2nd day after the peritoneal injections. DNA damages were determined by single cell gel electrophoresis(SCGE) and 3 H-TdR incorporation. Results Acquired by SCGE technique, tail movement of DNA in mercury-cadmium-lead group was significantly greater than that in the single exposure group, the difference was significant too beteen mercury-cadmium group and cadmium group, cadmium-lead group and cadmium group. The results of 3 H-TdR incorporation showed: the values of DPM in mercury-cadmium group and cadmium-lead group were lower than that in the single exposure group and the value of DPM lowerd more significantly after exposure to mercury-cadmium-lead. Conclusion The combined effects of mercury, cadmium, lead on DNA damage are more significant.

关 键 词：汞 镉 铅 联合染毒 DNA损伤 

分 类 号：R994[医药卫生—毒理学]