液质联用技术研究肽类药物药动学中的样品制备技术  

Sample preparation by using LC-MS in pharmacokinetic studies of polypeptide drugs

作　　者：戴舒佳[1] 汤仲明[1] 钱小红[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《中国新药杂志》2004年第11期984-987,共4页Chinese Journal of New Drugs

基　　金：国家863计划重大专项(2003AA2Z347B)国家自然科学基金重点项目(39930180)国家自然科学基金面上项目(20275004)

摘　　要：肽类药物的研究开发成为新生物技术药物研究开发的热点之一。然而,建立可靠、灵敏和准确的肽类药物体内分析方法是肽类药物临床前和临床药动学研究的难点。样品制备技术是建立符合药动学研究要求的定量分析方法的关键。现综述液质联用技术在肽类药物药动学研究中的样品制备技术方法的目的、要求、特点和分类。结合近年发表的文献具体阐述了固相萃取法、蛋白质沉淀法、超滤离心法、亲和层析法和部分其他方法的原理及其应用实例。The discovery of new polypeptide drugs is becoming one of the most favorable topics in the biotechnological medicine. It is considered as hard cores to develop reliable and sensitive quantitative analysis in vivo,as well as the sample preparation technology,of polypeptide drugs for the preclini-cal and clinical pharmacokinetic studies. This paper reviews the objectives, requirements, classification and characteristics of sample preparations by using liquid chromatography tandem mass spectrometry (LC-MS)in pharmacokinetic studies of polypeptide drugs. It also introduces the principles and applications of variety of sample preparation methods including solid phase extraction, protein precipitation, ultra-filtered centrifugation and affinity chromatography.

关 键 词：液质联用 肽类药物 药动学 样品制备 

分 类 号：R917.1[医药卫生—药物分析学] R969.1[医药卫生—药学]