枯草杆菌屎肠球菌二联活菌制剂对肝硬化大鼠肠道细菌易位的影响  

Effects of Bacillus subtilis and Enterococcu faecim on bacterial translocation in cirrhotic rats

作　　者：原庆[1] 孙天燕[2] 王惠吉[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]北京第六医院,北京100037

出　　处：《中国新药杂志》2004年第11期988-990,共3页Chinese Journal of New Drugs

摘　　要：目的:观察枯草杆菌屎肠球菌二联活菌制剂(商品名:美常安)对肝硬化大鼠肠道细菌易位的影响。方法:皮下注射40％ CCl4橄榄油溶液诱导大鼠肝硬化模型,用枯草杆菌屎肠球菌二联活菌制剂治疗。采用改良鲎试验测定血清内毒素,无菌操作取肠系膜淋巴结、肝、脾组织分别进行细菌培养观察细菌易位情况。结果:枯草杆菌屎肠球菌二联活菌制剂组的内毒素血症阳性率为35.29％,明显低于肝硬化组的73.33％(P<0.05);细菌易位率为47.05％,明显低于肝硬化组的93.33％(P<0.05)。结论:枯草杆菌屎肠球菌二联活菌制剂能够降低肝硬化大鼠血清内毒素,减少肠道细菌易位发生的机会。Objective:To evaluate the effects of Bacillus subtilis and Enterococcus faecim (Medilac-s) on bacterial translocation in cirrhotic rats.Method:The cirrhotic rats induced by subcutaneous 40% CCl4-olive oil solution were randomized to be treated with Bacillus subtilis (R-179) and Enterococcus faecim (R-026) or placebo for 14 days. At the end of treament,the plasma endotoxin was measured by limulus ameobocyte lysate (LAL) ,the mesenteric lymph nodes, tissue of spleen and liver were obtained. All samples were cultured in agar-blood plates. Results:The positive rate(35.29%) of plasma endotoxin in treatment group was significantly lower than that(73.33%) in model group(P< 0.05);the incidence(47. 05%) of bacterial translocation in treatment group was lower significantly than that(93.33%) in model group(P<0.05). Conclusion: The Bacillus subtilis (R-179) and Enterococcus faecim (R-026) could prevent the bacterial translocation and decrease the level of plasma endotoxin significantly in cirrhotic rats.

关 键 词：肝硬化大鼠 内毒素 细菌易位 微生态制剂 

分 类 号：R965.1[医药卫生—药理学] R392.11[医药卫生—药学]