复合RT-PCR快速鉴别鹅副粘病毒与鸡新城疫病毒  

Differentiation of Goose Paramyxov irus (GPV) and Newcastle Disease Virus (NDV) by Multiplex RT -PCR

作　　者：单松华[1] 邹键[2] 姚龙涛 胡永强[1] 何水林 龚祖埙[2] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]中国科学院生物化学与细胞生物学研究所,上海200031 [3]上海市奉贤畜牧兽医站,上海201400

出　　处：《上海交通大学学报（农业科学版）》2004年第4期355-357,365,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科技兴农重点攻关项目(2001)III-4

摘　　要：对新近分离的鹅副粘病毒SF02株通过自行设计的3条引物建立了复合RT-PCR方法。该方法对鹅源副粘病毒SF02株和GPV2株均可检测到215bp和401bp2条带,对12株(其中7株参考株,5株野毒)鸡源新城疫、2株鸽源新城疫、1株鸭源新城疫病毒的检测只出现1条401bp带,与预期的大小一致,对H5、H9亚型禽流感病毒、鸭病毒性肝炎病毒、传染性支气管炎病毒、SPF鸡胚尿囊液均未检测到特异性条带。A multiplex RT -PCR technique has bee n developed for differentiation of g oose paramyxovirus(GPV)and Newcastle disease virus.A set of three oligonucleotide primers(P3,P4and P5)were designed and used in this multip lex RT -PCR technique of GPV and NDV genomic RNA.The RT -PCR pr oduct of 215bp and 401bp were generated only with RNA from SF02and GPV2isolate orginated from goose,where as 401bp product only with RNA from 12NDV isolates(7reference strains and 5field isola tes)originated from chicken,2NDV isola ted from pigeon and 1NDV isolated fro m duck.However,the RT -PCR failed to detect avian influenza virus H5、H9subtype,duck viral hepatitis,in fectious bronchitis virus and the allantoic fluid of SPF chicken.

关 键 词：复合RT-PCR 鉴别诊断 鹅 副粘病毒 鸡 新城疫病毒 

分 类 号：S858.31[农业科学—临床兽医学] S858.33[农业科学—兽医学]