Mechanism of Inhibition to HIV-1 by Mycoplasma Fermentans  

作　　者：尚红 姜拥军 王琪 王亚男 张子宁 

机构地区：[1]AIDS Research Center, the First Affiliated Hospital, China Medical University, Shenyang 110001, China

出　　处：《Journal of Microbiology and Immunology》2003年第1期44-47,共4页中华微生物学和免疫学（英文版）

基　　金：This study was supported by a grant from the National Natural Science Foundation of China (No. 39770678)

摘　　要：To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purified with high performance liquid chromatography (HPLC).The inhibitory activities to reverse transcriptase (RT) and the nuclease activities were detected; the influence of M.fermentans on IL-10 secretion by both normal and HIV-1 infected human PBMC were determined, and the inhibitory effect of rhIL-10 on HIV-1 replication was detected with ELISA method. The results showed that the purified proteins with a molecular weight of 67-100 kDa or 10-25 kDa showed a 36% or 34% in hibitory activity to RT and partial nuclease activity. The thallodic protein could induce both normal and HIV-1 infected PBMC to secret IL-10 remarkably, and to the latter, this effect was more apparent. While rhIL-10 could inhibit replication of HIV-1 in PBMC in vitro in a dose-dependant manner. It concludes that the inhibitory effect of the M.fermentans PG18 culture supernatants on RT and the promoting effect of PG18 thallodic protein on IL-10 secretion in PBMC explain the mechanisms of inhibition to HIV-1 by M.fermentans PG18.To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purified with high performance liquid chromatography (HPLC).The inhibitory activities to reverse transcriptase (RT) and the nuclease activities were detected; the influence of M.fermentans on IL-10 secretion by both normal and HIV-1 infected human PBMC were determined, and the inhibitory effect of rhIL-10 on HIV-1 replication was detected with ELISA method. The results showed that the purified proteins with a molecular weight of 67-100 kDa or 10-25 kDa showed a 36% or 34% in hibitory activity to RT and partial nuclease activity. The thallodic protein could induce both normal and HIV-1 infected PBMC to secret IL-10 remarkably, and to the latter, this effect was more apparent. While rhIL-10 could inhibit replication of HIV-1 in PBMC in vitro in a dose-dependant manner. It concludes that the inhibitory effect of the M.fermentans PG18 culture supernatants on RT and the promoting effect of PG18 thallodic protein on IL-10 secretion in PBMC explain the mechanisms of inhibition to HIV-1 by M.fermentans PG18.

关 键 词：Mycoplasma fermentans HIV-1 IL-10 

分 类 号：R512.91[医药卫生—内科学]