脂多糖对血管平滑肌细胞表达MMP-2、9及TIMP-1、2的影响  

Effects of Lipopolysaccharides on Expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in the Cultured Rat Vascular Smooth Muscle Cells

作　　者：路军[1] 阮秋蓉[1] 朱大和[1] 瞿智玲[1] 任新瑜[1] 朱敏[1] 

机构地区：[1]华中科技大学同济医学院基础医学院病理学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第3期247-251,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No 39730 2 2 0 )

摘　　要：目的　探讨脂多糖 (lipopolysaccharides,LPS)对培养的大鼠血管平滑肌细胞 (vascularsmoothmusclecell,VSMC)表达基质金属蛋白酶 2、 9(matrixmetalloproteinase 2 , 9;MMP 2 ,MMP 9)及其组织抑制因子 1、 2 (tissueinhibitorofmatrixmetalloproteinases 1, 2 ;TIMP 1,TIMP 2 )的影响。方法　贴块法进行血管平滑肌细胞培养 ,细胞免疫化学检测基质金属蛋白酶及其抑制剂的蛋白表达 ,原位分子杂交分析MMP 9mRNA表达。结果　LPS是MMP 9的强诱导物 ,可在蛋白及mRNA水平诱导MMP 9的表达 ,同时LPS可诱导TIMP 1的表达 ,作用较对MMP 9减弱。LPS对二者诱导作用强度与LPS的浓度及作用时间呈正相关。LPS降低TIMP 2的表达 ,其表达与LPS的浓度及作用时间呈负相关。LPS对MMP 2的表达没有明显作用。结论　LPS诱导平滑肌细胞MMP 9、TIMP 1表达的同时可降低TIMP 2的表达 ,其作用呈浓度时间依赖性 ,在动脉粥样硬化发病过程中可能起一定作用。Objective To study the effects of lipopolysaccharides (LPS) on the expression of the MMP-2, MMP-9, TIMP-1 and TIMP-2 in the cultured rat vascular smooth muscle cells (VSMCs). Methods Rat VSMCs were cultured by a substrate-attached explanted method. The protein expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 was detected by immunohistochemistry and the expression of MMP-9 mRNA by hybridization in situ.Results Immunohistochemistry and hybridization in situ revealed that the protein and mRNA expression of MMP-9 was significantly increased. The change of TIMP-1 expression was the same as that of MMP-9. In contrast, LPS could decrease TIMP-2 protein production, but had no significant effect on the expression of MMP-2. Conclusion LPS induced the expression of MMP-9 and TIMP-1 in a dose- and time-dependent manner. LPS decreased the expression of TIMP-2. We concluded that LPS might play a role in atherosclerosis.

关 键 词：表达 LPS MMP-9 TIMP-1 血管平滑肌细胞 MMP-2 TIMP-2 MRNA水平 负相关 时间依赖性 

分 类 号：R735[医药卫生—肿瘤] R737[医药卫生—临床医学]