白松片对应激大鼠模型海马Glu、Asp、GABA含量的影响  

Effects of Baisong-Tablet on Levels of Aspartate, Glutamate and Gama-aminobutyric Acid in Hippocampus of Rat in Stress

作　　者：张春虎[1] 胡随瑜[1] 陈昌华[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所

出　　处：《中国心理卫生杂志》2004年第6期383-386,共4页Chinese Mental Health Journal

基　　金：国家"十五"科技攻关项目 (2 0 0 1 BA70 1 A2 2 )

摘　　要：目的 :探讨中药白松片对应激大鼠海马谷氨酸、天冬氨酸、γ-氨基丁酸含量的影响。方法 :将健康成年雄性大鼠 4 8只 ,随机分为 :正常对照、模型对照、氟西汀对照及白松片 3个剂量 6组 ,每组 8只 ,通过强迫游泳建立应激大鼠模型。采用预防加治疗给药 ,高效液相色谱仪 -荧光检测法检测大鼠海马 Glu、 Asp及 GABA含量。结果 :应激 7天后 ,模型对照组海马 Asp、Glu含量 (分别为 :1.5 2 2± 0 .2 94、5 .12 3± 0 .4 91μmol/g) ,高于正常对照组 ,差异有显著性 (P均 <0 .0 1) ;白松片等效、 3倍、 5倍剂量组海马 Asp、 Glu含量 [Asp分别为 :(1.2 6 8± 0 .2 37)μmol/ g,(1.2 5 3± 0 .2 4 0 )μmol/ g,(1.16 8± 0 .135 )μmol/ g,Glu分别为 (4 .0 5 1±0 .75 2 )μmol/ g,(3.5 6 5± 0 .6 71)μmol/ g (3.4 35± 0 .4 4 3)μmol/ g]均低于模型对照组 ,差异有显著性 ,(P<0 .0 5、P<0 .0 1)。结论 :白松片能降低应激大鼠海马 Asp、Glu的含量。Objective:To explore the effect of Baisong-Tablet on aspartate, glutamate and gama-aminobutyric acid levels in hippocampus homogenate of stress rat.Methods:48 adult Sprague-Dawley male rats were randomly divided into six groups including 3 control groups (Normal control ,Model control and Fluoxetine hydrochloride control) and 3 treatment groups (different dose of Baisong-Tablet treatment ). The stress model be established by forced swimming. The levels of aspartate, glutamate, and gama-aminobutyric acid were measured by reversed-phase high-performance liquid chromatography. Results:The levels of aspartate and glutamate in the model control group were significantly higher than that in normal control group respectively (all P<0.01).Compared with model control group, the levels of aspartate and glutamate in all BaiSong-Tablet treatment groups were significantly lower. (P<0.01or P<0.05). Conclusion:BaiSong-Tablet can decrease the levels of aspartate and glutamate acid in hippocampus of stress rat.

关 键 词：白松片 应激大鼠 海马 含量 对照组 鼠模型 GABA 结论 显著性 差异 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]