Enantiomeric Separation of Epinephrine and Salbutamol by Micellar Electrokinetic Chromatography Using β-Cyclodextrin as Chiral Additive  

作　　者：郑妍鹏 莫金垣 

机构地区：[1]School of Science, Beijing Jiaotong University, Beijing 100044, China [2]School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou, Guangdong 510275, China

出　　处：《Chinese Journal of Chemistry》2004年第8期845-848,共4页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (No. 29675033); the Natural Science Foundation of Guangdong Province(No. 001237) and Pandeng Foundation of Beijing Jiaotong University (No. PD245).

摘　　要：Enantiomeric separations of epinephrine and salbutamol, by means of micellar electrokinetic chromatography (MEKC) employing β-cyclodextrin as chiral additive in ammonium chloride-ammonia solution were investigated. In this system, the analytes migrated with the micellar phase towards the anode and were detected by electrochem- istry using gold microelectrode at +0.65 V vs. SCE. The success of the chiral separations is strongly dependent on the concentration of β-CD and SDS, and the optimal concentration is 8 mmol?L-1 and 15 mmol?L-1 respectively. The effects of detection potential, pH value of electrolyte and applied voltage were discussed also. Using the pro- posed method, baseline separation of the enantiomers could be accomplished in 6 min. Further, an attempt was made to elucidate the plausible mechanism of the chiral recognition.Enantiomeric separations of epinephrine and salbutamol, by means of micellar electrokinetic chromatography (MEKC) employing β-cyclodextrin as chiral additive in ammonium chloride-ammonia solution were investigated. In this system, the analytes migrated with the micellar phase towards the anode and were detected by electrochem- istry using gold microelectrode at +0.65 V vs. SCE. The success of the chiral separations is strongly dependent on the concentration of β-CD and SDS, and the optimal concentration is 8 mmol?L-1 and 15 mmol?L-1 respectively. The effects of detection potential, pH value of electrolyte and applied voltage were discussed also. Using the pro- posed method, baseline separation of the enantiomers could be accomplished in 6 min. Further, an attempt was made to elucidate the plausible mechanism of the chiral recognition.

关 键 词：micellar electrokinetic chromatography electrochemical detection enantiomer separation epineph-  rine SALBUTAMOL 

分 类 号：O657.7[理学—分析化学]