Elucidation of O-Phosphoryl and N-Phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spectrometry  

作　　者：张建臣 曹书霞 徐军 廖新成 赵玉芬 

机构地区：[1]The Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University Zhengzhou, Henan 450052, China [2]The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, De-partment of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Journal of Chemistry》2004年第8期870-873,共4页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (No. 20175026).

摘　　要：Mass spectroscopic characteristics of phosphoryl amino acids were studied in detail by positive and negative electrospray ionization mass spectrometry (ESI-MS) in conjunction with tandem mass spectrometry (MS/MS). Be- sides N-diisopropyloxyphosphoryl amino acids (N-DIPP-AA), O-phospho- and O-diisopropyloxyphosphoryl amino acids (O-DIPP-AA) were studied and compared to N-DIPP-AA. The fragmentation pathways of [M+H]+, [M+Na]+ and [M-H]- ions of phosphoryl amino acids were summarized. In addition to several similar patterns, each of them showed its characteristic fragmention.Mass spectroscopic characteristics of phosphoryl amino acids were studied in detail by positive and negative electrospray ionization mass spectrometry (ESI-MS) in conjunction with tandem mass spectrometry (MS/MS). Be- sides N-diisopropyloxyphosphoryl amino acids (N-DIPP-AA), O-phospho- and O-diisopropyloxyphosphoryl amino acids (O-DIPP-AA) were studied and compared to N-DIPP-AA. The fragmentation pathways of [M+H]+, [M+Na]+ and [M-H]- ions of phosphoryl amino acids were summarized. In addition to several similar patterns, each of them showed its characteristic fragmention.

关 键 词：N-phosphoryl amino acid O-phosphoryl amino acid electrospray ionization tandem mass spec-  trometry 

分 类 号：O657.63[理学—分析化学]