赤霉素介导模拟失重诱导的胡萝卜细胞中淀粉粒降解  

GIBBERELLINS MEDIATE THE DEGRADATION OF STARCH GRAINS INDUCED BY SIMULATED MICROGRAVITY IN CARROT CALLUS CELLS

作　　者：崔大勇[1] 蔡伟明[1] 汤章城[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《空间科学学报》2004年第5期394-400,共7页Chinese Journal of Space Science

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-SW-322);国家自然科学基金项目(39770199)共同资助

摘　　要：空间微重力环境会影响生命活动,本文利用回转器来模拟微重力(失重)的生物学效应.回转处理可以引起胡萝卜愈伤组织细胞中内源活性赤霉素(GAs)水平上升,总淀粉酶活力提高(其中主要是α-淀粉酶活力增加),淀粉粒降解.且GAs和α-淀粉酶活力的升高趋势一致.GAs合成性抑制剂,ancymidol能显著抑制回转处理中α-淀粉酶活力的增加.由此推测:回转处理中GAs的升高诱导了α-淀粉酶基因表达增加,后者促使淀粉粒降解和能量物质动员,以响应模拟失重的刺激.Microgravity environment in space affects organism. The biological effects of microgravity have been used a clinostat to simulate. The clinostat treatment induced the increase of gibberellins (GAs) and the activity of total amylase (especially the increment of a-amylase activity), as well as the degradation of starch grains in carrot callus cells. The increment trend of GAs and α-amylase activity was con-sistent. Further more, ancymidol, the inhibitor of the biosynthesis of GAs could remarkably repress the increment of α-amylase activity induced by the clinostat treatment. Therefore, we deduce that clinostat treatment can stimulate the raise of GAs, which induces the increment of the expression of α-amylase genes, then trig-gers the degradation of starch grains. As a result, plant can produce more active energy to respond to clinostat stimulus.

关 键 词：微重力 胡萝卜 赤霉素 淀粉酶 回转器 国际空间站 植物向重性研究 

分 类 号：S631.2[农业科学—蔬菜学]