应用SSR标记技术鉴定登海11号玉米杂交种纯度的研究  

Application of SSR Marker Technique to Purity Testing of Denghai No.11 Maize

作　　者：李群[1] 李汝玉[1] 颜廷进[1] 谭振新[1] 

机构地区：[1]农业部农作物种质监督检验测试中心,山东济南250100

出　　处：《种子》2004年第10期21-23,共3页Seed

摘　　要：以登海 1 1号玉米杂交种及其亲本和 9对玉米 SSR位点引物为材料 ,筛选出了适合该杂交种纯度鉴定的 SSR位点引物 ;并对利用幼苗和干种子为材料提取 DNA的效果进行了比较 ,建立了一套利用 SSR标记技术进行玉米杂交种纯度鉴定的技术规程。该操作程序简单、快速、对仪器设备要求较低 ,易于推广。In this study, 9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purity of Denhai No.11 maize hybrid, which lack a reliable laboratory method for purity testing. A polymorphic SSR loci, phil 053, has been found that could be applied to test the purity of this hybrid. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker.

关 键 词：SSR标记 技术鉴定 登海11号 玉米 杂交种 纯度 

分 类 号：S513[农业科学—作物学]