电位扫描过程中NAD^+在银电极上的原位表面增强拉曼光谱  

In-situ SERS Raman Spectra of NAD^+ on Silver Electrode Recorded during a Potential Scanning Procedure

作　　者：杨海峰[1] 封婕[1] 王桂华[1] 章宗穰[1] 

机构地区：[1]上海师范大学生命与环境科学学院化学系,上海200234

出　　处：《化学学报》2004年第20期2007-2009,F006,共4页Acta Chimica Sinica

基　　金：上海市教委高校青年科学基金 (No.98QN77);上海市教委发展基金 (No .0 3DZ1 6)资助项目

摘　　要：利用共焦激光拉曼系统 ,原位测定了电位扫描过程中NAD+ 分子在银电极上的表面增强拉曼光谱的变化 .通过分析0 .4 -0 .2 0 .4V电位区间的拉曼光谱的变化 ,推断由于NAD+ 分子中存在着具有空间旋转自由度的磷酸二酯键 。In-situ study of NAD(+) adsorbed on a chemically roughened silver surface has been conducted using surface enhanced Raman scattering method combined with a confocal technique. The recorded spectra depict that under polarization conditions from 0.4 to 0.2 V, the adenine moiety of NAD(+) adopts a perpendicular orientation via the N7 and amino group with respect to the silver surface. In the more negative potential range from 0.1 to -0.2 V, the adenine ring moiety tends to lay on the surface in a flat conjugation. In addition, the adsorption mode of the nicotinamide moiety also shifts with the potential scan. It could be concluded that the adsorption ways of both adenine and nicotinamide moieties are dependent of the applied voltages due to existing a flexible pyrophosphate bridging of them.

关 键 词：电位扫描过程 银电极 原位测定 表面增强拉曼光谱 NAD^+ 辅酶 共焦激光拉曼系统 

分 类 号：O657.3[理学—分析化学]