巯基苯类为标记分子免疫检测的FT-SERS光谱研究  

Study on Fourier Transform Surface-Enhanced Raman Scattering of Immunoassay Using Mercapto Molecules as Reporter-labels

作　　者：李淑瑾[1] 顾仁敖[1] 

机构地区：[1]苏州大学化学系,苏州215006

出　　处：《化学学报》2004年第20期2118-2122,F012,共6页Acta Chimica Sinica

基　　金：国家自然科学基金 (Nos.2 0 2 730 4 5 ;2 0 3730 4 6);厦门大学固体表面物理化学国家重点实验室开放基金资助项目

摘　　要：以对巯基苯甲酸、苯硫酚为标记分子 ,与金纳米粒子生成具有SERS信号的标记金溶胶 .在一定条件下 ,标记金溶胶与抗体羊抗小鼠IgG形成SERS标记免疫金溶胶 .用组装金纳米粒子的玻璃表面吸附抗体 ,通过对相应抗原小鼠IgG的识别 ,形成固相抗体 /抗原对 ,再将SERS标记免疫金溶胶组装到固相抗体 /抗原对上 ,形成“固相抗体 -待测抗原 -标记抗体(即标记免疫金溶胶 )”夹心复合物 。Gold colloids were labeled with Raman reporter mercapto molecules such as 4-mercaptobenzoic acid and thiophenol to form Fourier transform (FT) surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Firstly, antibody goat anti-rat was adsorbed onto the reporter-labeled gold colloids to form reporter-labeled immunogold colloids in appropriate condition. Secondly, the glass slide which was assembled gold nanoparticles was coated with antibody and selectively adsorbed antigen rat IgG to form antibody-antigen couple, and the SERS reporter-labeled immunogold colloids were modified onto the couple in a glass slide with gold nanoparticles adsorbing 'antibody/ antigen/reporter-labeled immunogold colloids' to form sandwich-type complex through the specific adsorption between antibody and antigen. The selective interaction between antibody and antigen can be read out by the labeling molecules.

关 键 词：标记分子 免疫检测 金纳米粒子 表面增强拉曼散射光谱 对巯基苯甲酸 苯硫酚 夹心复合物 

分 类 号：O657.3[理学—分析化学]