他克莫司影响朗格汉斯细胞迁移机制的研究  

The Mechanisms of Tcrolimus on the Migration of Langerhans' Cells

作　　者：唐玲[1] 于益芝[2] 陈洁[1] 阎明[1] 顾军[1] 郑茂荣[1] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]第二军医大学免疫学研究所,上海200433

出　　处：《中华皮肤科杂志》2004年第11期653-655,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30271202);国家863计划(2001AA215091);上海市卫生局科技发展基金(034113)

摘　　要：目的探讨他克莫司影响朗格汉斯细胞迁移能力的原因。方法用不同浓度的他克莫司处理人角质形成细胞株HaCaT,24h后收集上清液,通过ELISA法检测上清液中的单核细胞化学吸引蛋白质1(monocytechemoattractantprotein1,MCP-1)浓度,并通过RT-PCR法检测细胞中MCP-1mRNA水平。结果经质量浓度为625～5000ng/mL他克莫司处理过的HaCaT株,其分泌至上清液中的MCP-1水平明显受到抑制,细胞中MCP-1mRNA的水平低于正常对照组。结论他克莫司通过抑制角质形成细胞中MCP-1的分泌、表达,进而影响了朗格汉斯细胞前体及朗格汉斯细胞趋化至皮损处,阻止或减弱了某些免疫反应。Objective To investigate the mechanisms of tacrolimus on the migration of Langerhans′ cells. Methods The concentration of MCP-1 in the cultured supernatants of HaCaT cell treated with tacrolimus was determined by ELISA. The expression level of MCP-1 mRNA in HaCaT cell treated with tacrolimus was studied by RT-PCR method. Results The concentration of MCP-1 in HaCaT cell treated with 625 ～ 5 000 ng/mL of tacrolimus was significantly decreased. The expression level of MCP-1 mRNA was also markedly decreased. Conclusions Tacrolimus may probably decrease the expression of MCP-1 in keratinocytes and suppress the chemotactic ability of Langerhans′ cells precursor and Langerhans′ cells, thus inhibiting or diminishing certain local immunoreaction.

关 键 词：他克莫司 朗格汉斯细胞 MCP-1 MCP—1 角质形成细胞 上清液 影响 化学 迁移能力 水平 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]