雷公藤多苷对小鼠腹腔激活巨噬细胞杀伤活性和NO分泌影响的实验研究  

Effect of polyglycoside of Trip terygium wilfordi Hook.f.on cytotoxicity and NO Production in activated peritoneal elicited macrophage of mice

作　　者：任燕[1] 杨业金[1] 汪少娟[1] 何为[1] 韩玲[1] 王辉丽[1] 

机构地区：[1]华中科技大学同济医学院实验研究中心医学分子生物学开放实验室第一室,湖北武汉430030

出　　处：《中医药学刊》2004年第11期2017-2019,共3页Study Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30070899)

摘　　要：雷公藤多苷可以作为免疫抑制剂用于自身免疫性疾病的治疗,如子宫内膜异位症、哮喘、肝炎等,既可以抑制细胞免疫又可以抑制体液免疫。目的:探讨雷公藤多苷(TWP)对小鼠腹腔活化巨噬细胞杀伤活性和NO分泌的影响。方法:分离小鼠腹腔巨噬细胞,用脂多糖(LPS)激活,给予雷公藤多甙(TWP)进行共同孵育;分别以间接MTT法和Griess试剂检测巨噬细胞的杀伤活性和上清NO的含量变化。结果:雷公藤多苷能有效抑制小鼠腹腔激活巨噬细胞的杀伤活性以及NO的生成。Object: In order to explore the immune inhibition by Trip terygium wilfordii Hook. f. on peritoneal macrophage of humane endomertrsis, observers established mice cell model to test the cytotxicity function and secretion change of NO from mice peritoneal macrophage in vitro induced by LPS. Methods: Separate and purify the peritoneal elicited M (?) of mice,then culture in vitro with a series concentration of TWP,at the same time the macrophage are activated by LPS. Test the cutotoxicity of M (?) by MTT colorimetry and then the content of nitric oxide was explored by Griess reagent. Result:TWP (6.25 -100μg/1)could inhibit the production and NO production of macrophage in vitro induced by LPS. ( P < 0.01). Conclusion: The results which suggest that TWP can inhibit the cytotoxicity function and secretion change of NO from mice peritoneal macrophage in vitro induced by LPS are conductive for investigation of new therapeutic approaches for curing endometriosis.

关 键 词：雷公藤多苷 巨噬细胞 子宫内膜异位症 杀伤活性 NO MTT 

分 类 号：R931[医药卫生—生药学]