颈痛宁对颈椎间盘髓核炎症介质影响的实验研究  

Experimental study of Jingtongning Decoction on PGE_2、6 - K -PGF_1α、HE of degenerated cervical unclens pulposos

作　　者：林石明[1] 许书亮[2] 李兆文[1] 陈联源[1] 陈鲁峰[1] 郑玉堂[1] 杨源中[1] 周琳英[2] 

机构地区：[1]福建省漳州市中医院骨科,福建漳州363000 [2]福建中医学院骨伤系,福建福州350003

出　　处：《中医药学刊》2004年第11期2043-2046,共4页Study Journal of Traditional Chinese Medicine

基　　金：福建省教育厅基金项目(3201-200107)

摘　　要：目的:观察颈痛宁对实验性颈椎病的治疗作用,并探讨其作用机理。方法:28只6月龄新西兰兔随机分成模型组及正常对照组,其中模型组20只,正常对照组8只。模型组用彭氏和余氏两种方法相结合建立模型。术后8周将模型组分为颈痛宁组、颈复康组、生理盐水组。分别喂服颈痛宁、颈复康、生理盐水,正常对照组喂服生理盐水。喂药4周后处死取材,取颈5-6、小颈6-7髓核,匀浆后取上清液,行放射免疫法测PGE2和6-K-PGF1α,用酶联免疫法测HE含量。结果:颈痛宁组、颈复康组、生理盐水组髓核组织申PGE2、6-K-PGF1α及HE水平均不同程度升高,颈痛宁组和颈复康组与生理盐水组比较,P<0.01,有显著性差异;生理盐水组与正常对照组比较,P<0.01,有显著性差异;颈痛宁组与颈复康组比较,无显著性差异,P>0.05;但颈痛宁组和颈复康组PGE2含量与正常对照组比较,无显著性差异,P>0.05,而两组6-K-PGF1α及HE相比,P<0.05,仍存在显著差异。结论:退变颈椎间盘髓核中PGE2、6-K-PGF1α及HE水平明显升高,表明髓核炎症反应与颈椎病发病有关,颈痛宁能有效抑制髓核炎症反应,这可能是其治疗作用机理之一。Object: To investigate the treatment effect of Jingtongning Decoction (JTND) on cervical spondylosis. Methods: In this experiment, 28 white New Zealand rabbits were used and divided into 2 groups randomly, model group and control group. The animal moldels were created by Peng Baogan and Yu Jiakuo procedure. After 8 weeks later when the animal models were successfully, they were divided into 3 groups randomly, Jingtongning(JTN) group, Jingfukang( JFK) group and normal Saline group. The animals were killed in order to collect experimental samples after 4 weeks treatment. The content of some inflammatory mediators (prostaglandin E2, 6 - deto - prostaglandin F1α and HE) were measured. Results: There was significant difference between normal Saline group and control group in variation of the contents of PGE2, 6 - ketone - PGF1α and HE in the intervertebral disc of the animals ( P <0.01). There was significant difference between the normal Saline group and control group ( P < 0.01). They had not difference between JTN group and JFK group ( P > 0.05 ). There was significant difference in comparison among the two administered groups (JTN group and JFK group) and the control group of the contents of 6-K- PGF1α and HE ( P <0.05) , but they had not difference in the contents of PGE2. Conchusion: The content of PGE2, 6 -kelone -PGF1α and HE in the degeneration cervical intervertebral disc of the model animals were always higher, which may be relating to the degeneration of cervical vertebral, JTND can effectively control the production of the 3 inflammatory mediators.

关 键 词：颈椎病 髓核 炎症介质 颈痛宁 实验研究 

分 类 号：R681[医药卫生—骨科学]