双试剂酚红比色法全自动测定血清碳酸氢根方法学研究  

The methodological study of serum bicarbonate test by double-reagent phenol red colormetric method

作　　者：李和楼[1] 郭英艾[1] 

机构地区：[1]泰山医学院附属医院检验科,山东271000

出　　处：《国外医学（临床生物化学与检验学分册）》2004年第6期497-499,共3页Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine

摘　　要：目的　建立一种能适合各级实验室常规和急诊检测血清HCO-3 的方法。方法　应用自配双试剂建立两步终点测定法 ,可同时满足全自动和半自动手工测定 ,用日立 70 6 0生化仪对本方法进行方法学评价。结果　比色液吸收峰为 5 5 8nm ,线性范围可达 5 0mmol/L ,批内CV 3.16 % ,批间CV4 .5 5 % ,平均回收率 10 2 .3% ,与血气分析仪测定结果相关性良好 ,Y =1.0 0 5 9X - 0 .10 38,r=0 .9813(n =6 0 ) ,黄疸、脂血、溶血标本对结果无影响 ,健康人静脉血HCO-3 浓度 2 1.2～ 31.2mmol/L。结论　该方法操作简便、灵敏准确、试剂稳定、成本低廉 ,可用于血清碳酸氢根的常规分析 。Objective To set up a test method of HCO - 3,which can be used in labs of different levels.Methods Using self-made reagents to set up a test method of two-step terminal point,the method was methodologically evaluated with comparison of Hitachi 7060 Biochemical analyzer.Results The absorption climaxity of colormetrical reagent in this method was 558nm.The linearity of method was up to 50mmol/L,the precisions was 3.45% for wihtin-vapor analysis device(X),correlative formula:Y=1.005 9X-0.103 8,the correlation coefficient(r) was 0.981 3(n=60).Serions jaundice,hyperlipoidemia and hemolysis samples had no influence on the result,the density of HCO - 3 in venous blood of heathy person was 21-23mmol/L.Conclusion The method is simple,precise,stable and low-costly.It may be used in labs of different levels.

关 键 词：血清 双试剂 方法学研究 比色法 酚红 溶血标本 脂血 测定法 吸收 测定结果 

分 类 号：R446.1[医药卫生—诊断学]