抗氧化干预在粒细胞减少大鼠肺部铜绿假单胞菌感染中的治疗作用  

The effect of antioxidant treatment in pneumonia of granulocytopenic rats

作　　者：徐金富[1] 瞿介明[1] 何礼贤[1] 张静 李倬哲[1] 陈雪华[1] 潘珏[1] 李惠萍[2] 

机构地区：[1]复旦大学附属中山医院肺科,上海200032 [2]上海市肺科医院呼吸科

出　　处：《中华内科杂志》2004年第11期853-856,共4页Chinese Journal of Internal Medicine

基　　金：上海市卫生系统优秀学科带头人培养计划(98BR0 3 0 )

摘　　要：目的　观察抗氧化干预在粒细胞减少大鼠肺部铜绿假单胞菌感染中的治疗作用。方法　雄性SD大鼠 90只 ,分为 2组 ,A组 :ICH对照组 ,制作粒细胞减少模型 ;B组 :抗氧化干预组 ,先按A组方法制作粒细胞减少模型 ,半小时后腹腔注射N 乙酰半胱氨酸 (NAC) 15 0mg·kg-1·d-1,连续 7d ,接种细菌前半小时再注射 1次NAC。用铜绿假单胞菌标准菌株制作肺部感染模型。观察感染 0h、6h、9h、2 4h、4 8h和 72h的外周血白细胞、氧化 /抗氧化指标、肺组织细菌负荷、肺血管渗透性指标和肺湿干比 (W/D)、肺组织病理学改变等。结果　 2组大鼠感染前外周血白细胞均低于 4×10 9/L ,B组血清和肺组织中丙二醛水平明显低于A组 ,2组肺组织细菌负荷无明显差异 ,B组肺血管渗透性和肺W/D均明显低于A组 ,而超氧化物歧化酶水平明显高于A组。肺组织病理学显示 ,A组肺损伤严重 ,肺充血 ,出血明显 ,肺上皮细胞凋亡小体形成。结论　粒细胞减少大鼠肺部感染时抗氧化干预可减轻肺损伤 。Objective To evaluate the therapeutic role of antioxidant intervention in granulocytopenia rats with pseudomonas aeruginosa pneumonia. Methods 90 male Sprague-Dawley rats were randomly divided into two groups. Group A: the control granulocytopenia group, in which the granulocytopenia was induced by using cyclophosphamide and cortisone acetate. Group B: the antioxidant intervention group, in which the granulocytopenia model was the same as group A, while peritoneal injection of NAC 150 mg·kg -1 ·d -1 half an hour after the immunocompromised model was reproduced, and the injection was continued for a consecutive 7 days, and NAC was injected once more half an hour before bacterial tracheal inoculation. The model of pulmonary infection was established by using standard a strain of pseudomonas aeruginosa . The time-course of the following was observed 0 h before bacterial inoculation, and 6 h,9 h,24 h,48 h and 72 h after infection: the peripheral white blood cells, mortality, oxidant/antioxidant indexes, bacterial burden of lung tissue homogenate, pulmonary vascular permeability and lung wet/dry weight ratio, and the pulmonary histopathological changes. Results The peripheral white blood cells of both groups were less than 4×10 9/L. The concentration of superoxide dismutase in both serum and lung tissue in group B were higher than that in group A, while concentration of malondialdehyde in group B was lower than that in group A. Pulmonary vascular permeability and lung wet/dry ratio of group B were much lower than that of group A. There was no difference in bacterial burden of lung tissue between the two groups. Group B showed a lower mortality than group A(16.3% vs 23.4%).Lung histopathological observation showed that lung injury, pulmonary congestion and hemorrhage were more serious or obvious in group A as compared with group B. Apoptotic bodies were found in the lung epithelial cells of Group A.Conclusions Antioxidant intervention can alleviate lung injury in the granulocytopenia r

关 键 词：粒细胞减少 肺组织 干预 大鼠 肺部感染 治疗作用 抗氧化 铜绿假单胞菌感染 充血 细菌 

分 类 号：R563.1[医药卫生—呼吸系统]