诱捕分离土壤中的生防放线菌  

Traping and isolation of biocontrol actinomyces from soil

作　　者：宗兆锋[1] 郭小芳[1] 韩立荣[1] 赵克刚 

机构地区：[1]西北农林科技大学植保学院植保资源与病虫害治理教育部重点实验室,陕西杨凌712100 [2]西安市农业科学研究所植保站,陕西西安710061

出　　处：《西北农林科技大学学报（自然科学版）》2004年第11期19-22,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家863计划项目(2002AA24-5121);农业部农业结构调整重大技术研究专项(2002-08-03A)

摘　　要：　以灰葡萄孢(Botrytiscinerea)和黄枝孢(Fulviafulva)2种病原真菌作为靶标菌,利用皿内诱捕法从土壤中分离获得22株放线菌,经对7种靶标真菌进行皿内拮抗性测定结果表明,其中8株放线菌对部分病原真菌有很强抑制作用,占到分离菌株总数的47.1%,有较强抑制作用的放线菌有7株。有45%的菌株可以抑制苹果炭疽菌、茄子黄萎菌和大豆腐霉菌。菌株SC1,SC11和A1的发酵液对4种靶标菌的抑制作用最强,75%菌株的发酵液对灰葡萄孢有较强的抑制作用,菌株SC1,SF1和A3的发酵液对苹果灰葡萄孢的控制作用超过80%。22 strains of actinomyces with biocontrol potential were isolated and trapped from soil using target fungal pathogens Botrytis cinerea and Fulvia fulva on PDA plate.There were 47.1% of actinomycetes strains with strong inhibition to the most of seven tested pathogens and 7 actinomycetes strains with relative strong inhibition.Colletotrichum gloesporioides,Verticillium dahliae and Pythium aphanidermatum could be suppressed by 45% of actinomyces strains.B.cinerea,C.gloesporioides,F.oxysporum f.sp.niveum and T.roseum were inhibited strongly by fermentation broth of strain SC1,SC11 and A1.B.cinerea could be inhibited by 75% of strains when using fermentation broth.More than 80% control effect could been gained to B.cinerea on apple with fermentation broth of stain SC1,SF1 and A3.

关 键 词：诱捕分离 土壤 放线菌 生物防治 灰霉病 

分 类 号：S476.19[农业科学—农业昆虫与害虫防治] S154.383[农业科学—植物保护]