壳聚糖大鼠颅内组织相容性的初步观察  

Experimental Studies on the Intracranial Biocompatibility of Chitosan

作　　者：周涛[1] 周波[2] 黄书岚[2] 刘仁忠[2] 徐海涛[2] 何文[3] 孙静[4] 

机构地区：[1]广州军区武汉总医院普通外科,430072 [2]武汉大学人民医院神经外科,430060 [3]武汉大学人民医院药学部,430060 [4]武汉大学医学院统计学教研室,430072

出　　处：《北京生物医学工程》2004年第2期140-142,共3页Beijing Biomedical Engineering

摘　　要：本实验探讨了生物可降解的天然高分子缓释材料壳聚糖在脑组织中的生物相容性。以期为临床提供可用于颅内植入化疗的药物缓释高分子载体。我们以 32只SD大鼠为研究对象 ,随机分为实验组和对照组 ,分别植入壳聚糖和明胶海绵。观察术后的行为改变和 3,7,1 4 ,30天时的局部组织反应 (HE染色 )。观察发现所有动物无明显行为改变 ,术后 3,7,1 4 ,30天的组织学观察表明壳聚糖与明胶海绵有类似的异物反应。因此壳聚糖具有良好的脑组织生物相容性 ,并可安全的降解。Intracranial biocompatibility is essencial for a system of intersticial drug delivery in brain, we therefore evaluated the biocompatibility a biodegradable nature high polymer, chitosan in the rat brain. Thirty-two adult Sprague Dawley rats were assigned to one of two groups and underwent unilateral temporal lobe implantation of Chitosan or Gelfoam (absorable gelatin sponge).Local reaction of surrounding tissues of the transplants were observed with HE stain after the transplantation at 3,7,14 and 30 days. None of the animals showed any behavioral changes or neurological deficits suggestive of either systemic or localized toxicity from the biodegradable chitosan, all surving to the schechuled date of sacrifice. Chitosan evoked a well localized inflammatory reaction compatable to that of Gelfoam. The Chitosan has been shown in this study to be nontoxic and biocompatible in rat brain.

关 键 词：壳聚糖 大鼠 颅内组织 相容性 脑组织 化疗 

分 类 号：R318.1[医药卫生—生物医学工程]