山羊生长激素基因5′调控区的多态性分析  

Polymorphism Analysis of the Goat Growth Hormone Gene in the 5′ Regulatory Sequence

作　　者：李美玉[1] 闵令江[1] 孙国强[1] 潘庆杰[1] 沈伟[2] 王广莲 

机构地区：[1]莱阳农学院动物科技学院,山东莱阳265200 [2]西北农林科技大学动物科技学院,陕西杨陵712100 [3]曹县农业局,山东曹县274400

出　　处：《遗传》2004年第6期831-835,共5页Hereditas（Beijing)

基　　金：山东省中青年科学家科研奖励基金 ( 0 1BS43 )资助~~

摘　　要：以鲁北白山羊、引进波尔山羊、纯繁波尔山羊以及鲁北白山羊与波尔山羊的杂交一代、回交一代共计 2 74个个体为研究材料 ,用两对引物分别扩增山羊生长激素 (GH)基因 5′区的 2 6～ 2 39bp以及 2 2 5～ 4 2 9bp片段 ,扩增产物经SSCP分析发现均存在多态性。在 2 6～ 2 39bp片段上 ,波尔山羊及杂交后代以AA型个体占多数 ,而鲁北白山羊则BB型个体较多 ;在 2 2 5～ 4 2 9bp片段上 ,所有种群均以CC型个体较多。对两个片段的纯合型 (AA ,BB ;CC ,DD)分别克隆测序发现 :(1) 2 6～ 2 39bp片段上AA型在第 6 0位发生了C→T的突变 ,第 2 11位发生碱基C的丢失 ,(2 ) 2 2 5～ 4 2 9bp片段上 ,DD型存在 3处突变 ,分别为 2 6 4位由T→C ,2 92位由T→A ,372位由C→T。上述结果为首次实验证实山羊生长激素The polymorphism of goat growth hormone gene in 5′-region in 274 goats including Lubei white goat, introducted Boer goat, pure bred Boer goat , hybrid generation 1 of LuBei white goat and Boer goat , backcross of hybrid generation 1 and Boer goat were detected by PCR-SSCP with two different pairs of primers. The result showed that in 26～239bp most of the Boer goats and hybrid generation presented as AA genotype,while most LuBei white goats presented as BB genotype. In 225～429bp all breeds presented as more CC genotype.The amplified fragments of AA,BB and CC,DD of the two fragments were cloned and sequenced . The result showed that in 26～239bp AA genotype had one substitution mutation at 60 site(C→T), and base C losing at 211 site and DD genotype had three substitution mutations at 264 site(T→C),292 site(T→A) and 372 site(C→T) in 225～429bp。The results above-mentioned first confirmed that there were polymorphisms in 5′ region of GH gene.

关 键 词：山羊 生长激素基因 PCR—SSCP 遗传多态性 

分 类 号：S827[农业科学—畜牧学]