用微卫星标记技术对国内BALB/c小鼠遗传质量的分析  

Analyzing Genetic Quality of BALB/c Mouse Strainsin China by Microsatellite Marking

作　　者：陈振文[1] 欧阳兆和[2] 董罡[2] 李瑞生[2] 

机构地区：[1]首都医科大学实验动物科学部,北京100054 [2]军事医学科学院实验动物中心,北京100071

出　　处：《遗传》2004年第6期845-848,共4页Hereditas（Beijing)

摘　　要：为了解和掌握国内BALB/c小鼠遗传质量状况 ,验证微卫星标记技术在近交系小鼠遗传检测中应用的可靠性 ,应用所筛选的小鼠不同染色体上的 14个微卫星基因座 ,通过PCR扩增对北京、上海、沈阳、广州、长春、重庆和哈尔滨 7个地区 11个厂家提供的BALB/c小鼠进行遗传质量分析。结果北京、上海、哈尔滨及广州地区 7家BALB/c小鼠在 14个基因座均呈现一条清晰条带 ,且群体间呈单态性。沈阳、广州、长春和重庆 4个群体有 8个基因座在群体内表现杂合或呈多态性 ;其中沈阳和长春分别在 1个基因座上表现多态性和杂合 ;广州另一群体有 4个基因座出现杂合或多态性 ;重庆群体有 7个基因座表现为杂合或多态性 。Eleven BALB/c mouse strains of Beijing,Shanghai,Shenyang,Haerbin, Guangzhou ,Chongqing and Changchun were monitored in order to assure the genetic quality of inbred BALB/c mouse strains in China and to estimate the credibility of microsatllite markers. Fourteen microsatellites loci on different chromosomes were investigated by PCR analysis.It showed that all these microsatellites DNA loci displayed single allelic gene band in mouse strains of Beijing,Shanghai and Haerbin.But the mice came from Shenyang, Guangzhou,Chongqing and Changchun had polymorphisms or heterozygosis,among which the Shenyang and Changchun strains showed polymorphisms and heterozygosis at two separate loci. Four loci showed polymorphisms or heterozygosis in one of the Guangzhou mouse strains. The Chongqing strains showed polymorphisms and heterozygosis at seven loci,including the D10Mit180 locus as compared with the Shanghai strains.

关 键 词：BALB/C小鼠 微卫星标记 遗传质量检测 

分 类 号：Q953[生物学—动物学]