水稻凋亡基因rPDCD5的克隆和表达分析  

The Cloning and Expression of a NovelrPDCD5 Gene from Rice

作　　者：米丽萍[1] 卫春[1] 黄俊生[2] 杜喜玲[1] 钱晓茵[1] 李克贵[1] 申国安[1] 杨金水[1] 

机构地区：[1]复旦大学生命科学院遗传研究所,上海200433 [2]华南热带农业大学中国热带农业科学院,海口571101

出　　处：《遗传》2004年第6期893-897,共5页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划资助项目 ( 2 0 0 1CB10 880 5 )~~

摘　　要：从水稻愈伤组织抑制消减杂交 (suppressionsubtractivehybridization ,SSH)文库中分离出一个PCD相关基因的EST片段 ,根据水稻基因组的序列设计引物 ,从汕优 6 3中分离与克隆出rPDCD5的全长cDNA。rPDCD5包含387bp的可译框 ,编码由 12 8个氨基酸构成的蛋白质。序列比对分析显示 ,该检测的蛋白质与已知PDCD5的高度同源。半定量实时PCR分析证实了该基因在环境因子 (低温处理和NaCl处理 )An EST related to the gene PCD was isolated from SSH (suppression subtractive hybridization) library of callus tissues of rice (Oryza sativa L.). Primers were designed to obtain its complete cDNA encoding putative apoptosis-related protein from Shanyou 63 (Oryza sativa L.). Sequencing indicated that the gene contained a 387 bp open reading frame, which encodes a protein containing 128 aa. Sequence alignment showed that the deduced protein is highly homologous to the known PDCD5. Real time quantitative PCR was performed to reveal that rPDCD5 was up-regulated in abiotic stress (low temperature and NaCl treatment).

关 键 词：程序性细胞死亡 凋亡 RT—PCR rPDCD5 适时PCR 

分 类 号：S511[农业科学—作物学]