两种蓝藻超低温保存抗冻保护剂的研究  

A STUDY OF ANTI-FREEZE PROTETIVE MATERIAL OF CYANOPHYTA

作　　者：闫立强[1] 赵树仁[1] 程爱华[1] 王起华[1] 

机构地区：[1]辽宁师范大学生物系

出　　处：《辽宁师范大学学报（自然科学版）》1993年第2期153-155,共3页Journal of Liaoning Normal University:Natural Science Edition

摘　　要：以两种蓝藻为试材,对影响超低温(液氮温度-196℃)保存成活率的保护剂及其配比进行了探讨,发现非渗透保护剂(蔗糖)比渗透性保护剂(DMSO)效果好,混和保护剂与单一蔗糖为保护剂的处理效果未见区别,由两种方法所测成活率看鱼腥藻(Anabaena sp.)比钝顶螺旋藻(Spirulinaplatensis)的抗寒性高.This study uses two kinds of Cyanophyta(Anataena sp.,Spiralina platensis as test material to inquire into protective material influencing Cryopreservation's(liquid nlirogen's tempera- ture is-196 C)survival rate and its preecription and rate.It is found that the protective material of non-osmosis(sucruse)has better effect than of asmosis(DMSO),there is no difference between the effects of mixing protective material and single su■ose protective material the surrival rate of two kinds of methods also shows that Anabaera sp.has higher frost resistance than Spiralina platensis.

关 键 词：超低温保存 抗冻保护剂 鱼腥藻 钝顶螺旋藻 抗冻性 

分 类 号：Q949.22[生物学—植物学]