胚珠培养获得秘鲁番茄与栽培番茄种间杂种具有非温敏抗根结线虫基因Mi-3  

Interspecific F_1 Hybrids via Ovule Culture Between L. peruvianum and L. esculentum Introgressed Mi-3 Gene, Resistance to Temperature Insensitive Root-Knot Nematode

作　　者：赵泓[1] 柴敏[1] 于栓仓[1] 刘凡[1] 姜立纲[1] 毛爱军[1] 

机构地区：[1]北京市农林科学院蔬菜研究中心,北京100089

出　　处：《分子植物育种》2004年第6期833-838,共6页Molecular Plant Breeding

基　　金：北京市科技新星计划项目资助(项目编号:2004B21)。

摘　　要：以含有Mi-3抗根结线虫基因的秘鲁番茄(Lycopersiconperuvianum)LA2823为父本,栽培番茄(Lycop-ersicon esculentum)高代优良自交系为母本,授粉后20～30d摘取幼果.将从幼果中分离出的直径大于2mm的胚珠放置在营养分化培养基上培养.2～4周后胚芽、胚根分别由两端伸出.培养的胚珠还可以通过体细胞胚发生途径产生胚芽.获得的种间杂种对根结线虫的抗性表型与野生种父本完全相同,在植物学性状上亦与其父本秘鲁番茄更相近.以栽培番茄为母本进行回交,成熟果实与母本相似,但其种子即使放置在MS培养基上也不能萌发.对回交种子胚败育现象进行了探讨.Wild tomato (Lycopersicon peruvianum Mill.) LA2823 carrying Mi-3 gene, was selected as the male parent for crosses with cultivated tomato (Lycopersicon esculentum Mill.) in order to introgression root-knot nematode resistance to the female parent, which was an inbred line possessing many excellent traits. Young fruits were collected at 20~30 days after pollination. All ovules bigger than 2 micrometer were removed out of the fruits and cultured on the differential medium for 2 to 4 weeks. Few of them germinated while some others produced germinal buds via somatic embryogenesis. Most of morphological characters of the putative F1 hybrids were more similar to their male parent; especially the resistance to root-knot nematode was the same. Two hybrid plants were backcrossed with L. esculentum to generate BC1 progeny. Mature fruits of BC1 were quite similar to their female parent, but all of their seeds could not germinate even cultured on MS medium. Abortion of postfertilization hybrid embryo in interspecific backcross was discussed.

关 键 词：胚珠培养 秘鲁番茄 栽培番茄 种间杂种 非温敏抗根结线虫 基因 种子胚败育现象 

分 类 号：S641.2[农业科学—蔬菜学]