黄连花皂苷类成分HPLC-ELSD法分析  

Analysis of the Saponins Isolated from Lysimachia davurica L. by HPLC - ELSD

作　　者：田景奎[1] 徐丽珍[1] 邹忠梅[1] 张宏武[1] 穆红梅[1] 杨世林[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《药物分析杂志》2004年第6期577-580,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(39870085)国家"九五"攻关课题(96-901-06-37)

摘　　要：目的:建立黄连花皂苷类成分HPLC分析方法。方法:应用HPLC-ELSD对黄连花分离得到的9种皂苷进行分析测定,以Zorbax SB-C18(4.6 mm×150 mm,5μm)为分析色谱柱,以乙腈-水(含0.2%醋酸)为流动相梯度洗脱,流速1 mL·min-1,蒸发光散射检测器,雾化气体流速3.35 L·min-1,漂移管温度117℃。结果:9种皂苷能较好地分离,通过对黄连花全草进行分析,表明Primulanin和黄连花皂苷L(davuricoside L)含量较高。结论:HPLC-ELSD适合没有紫外吸收或紫外吸收弱的皂苷类成分的分析。Objective: To establish a method for quantitative analysis of the saponins in Lysimachia davuria L. Methods: HPLC - ELSD was used for analyzing 9 saponins isolated from Lysimachia davurica L. , with the Zorbax SB - C18 column, 5 μm (4.6 mm×150 mm ) , and acetonitrile - water (contained 0. 2% HAc) gradient elution, flow rate 1. 0 mL·min-1 . Results:9 saponins were isolated. Through analyzing whole herb of Lysimachia davurica L. , contents of primulanin and davuricoside L were higher than others. Conclusion: HPLC - ELSD can be applied to analyze constituents with no UV absorption.

关 键 词：黄连花 皂苷类成分 HPLC—ELSD 黄连花皂苷 中药 

分 类 号：R284.1[医药卫生—中药学]