高效液相色谱法测定淡豆豉药材中异黄酮的含量  

HPLC Determination of Isoflavones Contents in Semen Sojae Preparation

作　　者：毛俊琴[1] 黄晓瑾[1] 周月芬[1] 宓鹤鸣[2] 

机构地区：[1]中国人民解放军第85医院,上海200052 [2]第二军医大学药学院药物分析教研室,上海200433

出　　处：《药物分析杂志》2004年第6期587-590,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法测定淡豆豉中异黄酮含量。方法:采用HPLC法,色谱柱为YWG C18柱(250 mm×4.5 mm,10μm),以甲醇-水-乙酸(10:10:1)为流动相,流速为0.8 mL·min-1,检测波长260 nm,测定淡豆豉中染料木素(genistein)、大豆黄素(daidzein)含量。结果:染料木素及大豆黄素的线性范围分别为2.00-40.00μg·mL-1和2.64-52.70μg·mL-1,相关系数分别为0.9994和0.9993,不同产地的淡豆豉中异黄酮含量差异较大。结论:本法准确、简便、快速,对于控制和评价淡豆豉的品质有重要意义。Objective: To establish a method for evaluating the contents of the isoflavones in Semen Sojae Preparation ( SSP). Methods: Contents of the isoflavones ( genistein and daidzein) in SSP was measured by HPLC [ a column of YWG C18 (250 mm×4. 5 mm, 10μm), methanol - water - acetic acid (10: 10: 1)as a mobile phase, flow rate 0. 8 mL·min-1, detective wave 260 nm]. Results: The calibration curves showed good linearity in the range of 2.00 - 40. 00μg·mL-1 and 2. 64 - 52. 70μg·mL-1, r = 0. 9994 and 0. 9993 respectively. There were obvious differences between the products of different habitats. Conclusion: The method is simple, sensitive, rapid, and can be used for the quality control of Chinese medicine SSP.

关 键 词：高效液相色谱法 测定 淡豆豉药材 异黄酮 含量 

分 类 号：R284.1[医药卫生—中药学]