中药丸剂中水分的GC法测定  

GC Determination of Water in Pills of Chinese Medicines

作　　者：贾敏鸽[1] 王燕[1] 许丹[1] 孙文基[1] 

机构地区：[1]西北大学生物医药重点实验室

出　　处：《药物分析杂志》2004年第6期616-619,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:对中药丸剂中水分含量用GC法进行了测定,考察了影响水分测定的多种因素。方法:采用甲苯法和GC法比较,GC法使用热导检测器(TCD),401有机担体柱(3 m×3 mm,80-100目),柱温:145℃,检测器温度:200℃,进样口温度:200℃,载气:氮气,流速30 mL·min-1。结果:GC法水的线性范围为2.151×10-3-2.151×10-2μg·μL-1(r=0.9993)。结论:气相色谱法测定快速、灵敏,成本较低,不污染环境。提供中国药典中成药蜜丸中水分测定法的修订参考。Objective:To establish a GC method for the determination of water in pills and investigate many factors effecting the content of water in pills. Methods: Both toluene method and GC method were used to determine the content of water in pills. In GC method,pills were dealt with absolute ethanol,1μL the solution was injected on a 401 organic support column(3 m ×3 mm,80 -100 mesh). The thermal conductivity detector(TCD)was used and detector temperature was 200℃. The injector port and detector temperature were 145℃ and 200℃ respectively. Carrier gas was nitrogen with a flow rate of 30 mL ·min-1. Results: A good linearity was obtained in the range of 2. 151×10-3 - 2. 151 ×10-2μg ·μL-1 ( r = 0. 9993). Conclusion: GC method was shown to be sensitive, fast, simple and without any pollution to environment, and cost less for determination of water content in large number of samples. A reference method for determination of water content in pills is set up for the recension of People's Republic of China Pharmacopoeia.

关 键 词：中药 丸剂 水分 GC法测定 甲苯法 

分 类 号：R286.0[医药卫生—中药学]