一种有效的葡萄叶片总RNA提取方法  

An Effective Method of Extraction Total RNA from Grape Leaves

作　　者：鲁晓燕[1] 赵英 孙文英[1] 

机构地区：[1]新疆石河子大学园艺系,新疆石河子832003 [2]新疆玛纳斯县人事局,新疆玛纳斯832200

出　　处：《新疆农业科学》2004年第6期456-457,共2页Xinjiang Agricultural Sciences

基　　金：新疆石河子大学

摘　　要：RNA的提取技术是现代分子生物技术的基础,植物RNA的提取比较困难。实验以新鲜的葡萄叶片为材料,采用SDS、CTAB相结合的方法,进行酚/氯仿的提取,消除了DNA及植物中较多的多糖、多酚等污染,提取到较纯净的总RNA,此方法适合于葡萄叶片总RNA的提取,试剂简单经济,实验结果稳定,重现性强。Extraction of RNA is a base of modern moleculor technology.It is difficult to extract RNA of plant. In this experiment ,fresh grape leaves are chosen as materials, SDS,CTAB and phenol/ccl4 are used to extract total RNA, to eliminate pollution from polysaccharide etc. .The method is suitable for extracting total RNA from grape leaves.It is simple, inexpensive, stable and easily repeated.

关 键 词：葡萄 叶片 总RNA 提取方法 SDS CTAB 酚/氯仿 

分 类 号：S663.1[农业科学—果树学]