IBDV不同分离株VP2高变区的基因序列分析  

Analysis of Gene Sequence on Different IBDV Strains' VP2 Variable Region

作　　者：白文霞[1] 鲍恩东[1] 侯继波[2] 何孔旺[2] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]江苏省农科院农业部畜禽疫病诊断重点开放实验室,江苏南京210014

出　　处：《四川畜牧兽医》2004年第12期26-27,共2页Sichuan Animal & Veterinary Sciences

摘　　要：对9个IBDV(传染性法氏囊病毒)分离株的VP2高变区进行RT-PCR扩增、测序,得到约560bp长的片段。分析比较9个IBDV分离株与参考毒株的VP2高变区(AccI～SpeI)的氨基酸序列,并构建进化树。结果表明,9个IBDV分离株是超强毒株,并且得出亚洲目前流行的vvIBDVs与欧洲国家的vvIBDVs的进化关系很近。Nine IBDVs were isolated from China and some referenced strains were involved in the experiment. Sequences of 560bp that containing VP2 gene were amplified by Reverse Transcriptase-Polymerase. The amino acid sequences of the9 isolated strains were compared to the referenced sequences with the aid of DNAstar software and analysis of the phylogenetic tree was performed. Sequences comparison and phylogenetic analysis indicated that the studyed IBDVs belonged to vvIBDVs which were prevalent in Asia and were similar to vvIBDVs in Europe.

关 键 词：传染性法氏囊病毒 变异毒株 致弱毒株 基因序列 进化 

分 类 号：S852.65[农业科学—基础兽医学]