11,12-环氧二十碳三烯酸对未成熟兔离体心脏停搏及再灌注性心律失常的影响  

Effects of11,12-epoxyeicosatrienoic acid on cardioplegia and reperfusion arrhythmias in the isolated immature rabbit hearts

作　　者：周明阳[1] 吴清玉[1] 钟星[2] 龙村[3] 温复兴[3] 覃筱燕[4] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院外科,北京100037 [2]中国医学科学院肿瘤研究所免疫室 [3]中国医学科学院中国协和医科大学麻醉与体外循环研究室,北京100037 [4]中央民族大学生物化学系

出　　处：《中华外科杂志》2003年第10期781-784,共4页Chinese Journal of Surgery

摘　　要：目的　观察 11,12 环氧二十碳三烯酸 (11,12 EET)对冷停搏未成熟兔离体心脏停搏效果及再灌注性心律失常的影响 ,探讨其作用机制。　方法　将 16只未成熟兔随机配对 ,分成对照组(离体心脏灌注St.Thomas Ⅱ停搏液 ,15ml/kg)和实验组 (离体心脏灌注含 70nmol/L11,12 EET的St .Thomas Ⅱ停搏液 ,15ml/kg)。利用非循环式Langendorff灌注装置 ,测定 (1)心脏灌注停搏液后的电机械活动停止时间和再灌注后电机械活动恢复时间 ;(2 )心脏停搏 2h(15℃ )后再灌注 1h(3 7℃ )过程中的心率、冠状动脉流量变化、心律失常活动及评分 ;(3 )心脏再灌注 1h后的心肌含水量、心肌钙离子含量。　结果　实验组与对照组的电活动停止时间 [(9 3± 0 9)s与 (13 6± 1 9)s ,P <0 0 1]、机械活动停止时间 [(4 5± 1 7)s与 (7 3± 2 1)s,P <0 0 5]、心肌钙离子含量 [(3 2 2± 0 3 3 ) μmol/克干重心肌 (gdw)与 (3 97± 0 2 6) μmol/gdw ,P <0 0 1]、心律失常发生及评分 [(2 0 3± 0 83 )与 (3 88± 1 2 5)分 ,P <0 0 1]、心肌含水量 [(84± 4)与 (90± 5) % ,P <0 0 1]比较 ,实验组均低于对照组。各时点冠状动脉流量显著增高。两组之间的电机械活动恢复时间、心率变化差异无显著性 (P >0 0 5)。Objective The aim of the present study was to study the Effects of 11,12 epoxyeicosatrienoic acid (11,12 EET) on cardioplegia and reperfusion arrhythmias in the isolated perfused immature rabbit hearts. Methods Isolated immature rabbit hearts were randomly divided into two groups: group 1 (St.Thomas No.2 solution control n=8) and group 2 (St.Thomas No.2 solution plus 11,12 EET n=8). By means of Langendorff technique, these isolated rabbit hearts underwent (15℃) hypothermia, 2 hours of ischemia after infusion of cardioplegic solution and 1hour of reperfusion (37℃). The mean times until the cessations of both electrical and mechanical activity were measured after infusion of cardioplegia. The same index until occurrence of both electrical and mechanical activity after reperfusion was observed too. We also measured the arrhythmias score, heart rate, coronary blood flow during the reperfusion and the myocardial water content, myocardial calcium content at the endpoint of the reperfusion period. Results The times until electrical [(9 3±0 9)s vs (13 6±1 9)s, P<0 01] and mechanical [(4 5±1 7) vs (7 3±2 1)s ,P<0 05] activity arrest were significantly shorter in the group 2 than those in the control group. 11,12 EET also provided significantly better myocardial water content [(84±4)% vs (90±5)%, P<0 01], arrhythmia scores (2 03±0 83 vs 3 88±1 25,P<0 01),coronary blood flow and myocardial calcium content [(3 22±0 33) μmol/gram dry weight (gdw) vs (3 97±0 26) μmol/gdw,P<0 01] compared with control. There were no significant changes with heart rate and the mean times until occurrence of both electrical and mechanical activity after reperfusion. Conclusions These data suggest that 11,12 EET added to the cardioplegic solution of St.Thomas No.2 has better cardioplegia effects and lower incidence of reperfusion arrhythmias.

关 键 词：未成熟 离体心脏 停搏液 再灌注性心律失常 对照组 心肌钙 冠状动脉 电活动 果实 配对 

分 类 号：R654.2[医药卫生—外科学]