应用硝酸纤维薄膜RIA对棘球蚴病、弓形虫病、丝虫病检测的研究  

EVALUATION OF A RADIO-IMMUNO-ASSAY (RIA) USING NITROCELLULOSE FILTER AS SOLID CARRIER FOR DIAGNOSIS OF HUMAN AND OVINE HYDATIDOSIS, PORCINE TOXOPLASMOSIS AND HUMAN FILARIOSIS

作　　者：方军[1] 匡兴伟[1] 吴兆敏[2] 承艺芳[2] 

机构地区：[1]上海中医学院同位素室 [2]中国农科院上海家畜寄研所

出　　处：《中国兽医寄生虫病》1993年第1期5-9,共5页Chinese Journal of Veterinary Parasitology

基　　金：国家自然科学基金

摘　　要：硝酸纤维薄膜作为RIA的一种新型固相载体,具有简易、快速、经济、易于漂洗、敏感性、特异性、重现性好等优点,且适用于大批量样品在短期内同时检测。可作为寄生虫病早期诊断可靠的新手段。该法已成功地应用于日本血吸虫病人、病牛及现场疫区耕牛的检测试验。本实验进一步对棘球蚴病人和病羊的血清;弓形虫病猪血清和丝虫病人血清等3种人畜共总寄生虫病进行检测研究,结果除对羊棘球蚴病检测效果不够理想,阳性检出率仅50％(10/20),其它几种疾病的检出率均在不同《度上高于ELISA和IHA法。对人棘球蚴病检测的阳性率,膜片法为100％(82/82),ELISA为91.5％(75/82),IHA为89.6％(71/82)。对猪弓形虫病的检测阳性率,膜片法为100％(120/120),ELISA为93.3％(112/120),IHA为90.0％(108/120)。对人丝虫病的阳性检出率膜片法为100％(80/80),ELISA为96.3％(77/80),IHA为88.9％(71/80)。各组假阳性率的降低更加明显。作者认为,该法可提供作为兽医寄生虫病早期大规模检测的一种有效方法。82 cases of human hydatidosis w, th 30 negative cases,29 cases of sheep hydatidosis with 20 negtivecases,120 cases of porcine toxoplasmosis with 60 negative cases, and also 80 cases of human filariosis with 20 negative cases were evaluated with RIA using nitrocellulose filter (NF-RIA) as its solid carrier, in comparison with conventional ELISA and IHA. The results showed that the NF-RIA had higher sensitivity and specificity than those of ELISA and IHA with the, cxception of sheep hydatidosis ,(rate of detection only 50%). The NF-RIA was there- fore considercd to be a simple, rapid, and sensitive method for diagnosis of the above mentioned human and animal parasitic diseases.

关 键 词：硝酸纤维薄膜 RIA 棘球蚴病 弓形虫病 丝虫病 诊断 固相放射免疫 

分 类 号：S855.91[农业科学—临床兽医学] S854.43[农业科学—兽医学]