一种诊断猪弓形虫病的新方法──碘标对流免疫电泳法  

A SIMPLE, RAPID, SENSITIVE NEW METHODRADIO-COUNTER-IMMUNOELECTROPHORESIS USING (125)￣I LABELLED ANTIGEN

作　　者：吴兆敏[1] 黄兵[1] 方军[1] 匡兴伟[1] 

机构地区：[1]中国农科院上海家畜寄研所,上海中医学院同位素室

出　　处：《中国兽医寄生虫病》1994年第3期6-9,共4页Chinese Journal of Veterinary Parasitology

基　　金：国家自然科学基金

摘　　要：作者报导用碘标记弓形虫病小鼠腹腔液分泌抗原对流免疫电泳新方法（ＲＣＩＥＰ）直接检测受检猪特异性抗体。ＲＣＩＥＰ与常规间接血凝（ＩＨＡ）对５０例病猪血清和５０例健康猪血清进行对比试验，结果证实该法具有高度的敏感性、特异性和较好的重现性。在不同稀释度时，弓形虫病猪血清ＲＣＩＥＰ和ＩＨＡ的阳性率分别为９４～１００％（平均９８．８０％）和２２～１００％（平均５８．８０％），健康猪的假阳性率前者未出现，而后者有１０％以上。从患病猪血清和抗原稀释度试验证实，ＲＣＩＥＰ的敏感性较酶标对流（ＥＬＣＥＰ）和常规ＣＩＥＰ分别高１５倍和４００倍。因此作者认为ＲＣＩＥＰ可作为猪弓形虫病的一种特异诊断新方法。The authors reported a new method of radio-counter-immunoeletrophoresis RCIEP using labelled secretory antigens of Toxoplasma gondii purified from ascitic fluid of mice and compared the results of RCIEP with that of IHA. 50 pigs inoculated with T. gondii and 50 healthy control pigs were used. The results showed high sensitivity, specifity and a good reproducibility of RCIEP. The positive rates were 94-100% (average 98.80%) with RCIEP in different dilutions of sera from toxoplasmasis cases. and 22-100% (average 58.80%) with IHA. No positive was observed in healthy individuals with RCIEP,while more than 10% false positive rate were appeared with IHA. The dilution test of sera from swine Toxoplasmasis and secretory antigen revealed that RCIEP was 15 times and 400 times more sensitive than ELCIEP and CIEP. Therefore. the RCIEP was considered to be a specific method for the toxoplasmasis diagnosis.

关 键 词：诊断 猪 弓形虫病 碘标对流免疫电泳 RCIEP 

分 类 号：S858.28[农业科学—临床兽医学]