硫芥淋巴细胞毒性作用与caspase-3表达  

Cytotoxic action of sulfur mustard on cultured lymphocytes and their caspase-3 expression

作　　者：赛燕[1] 刘勇[1] 朱明学[2] 张黎明[2] 董兆君[1] 

机构地区：[1]第三军医大学预防医学系军事毒理学教研室,重庆400038 [2]第二军医大学海医系,上海200433

出　　处：《第三军医大学学报》2003年第13期1127-1130,共4页Journal of Third Military Medical University

基　　金：全军"十五"重点课题 ( 0 1L0 54 )

摘　　要：目的　探讨硫芥对淋巴细胞的毒性作用方式和分子机制。方法　利用硫芥诱导的体外培养淋巴细胞 ,采用荧光分光光度法检测DNA的损伤 ,运用逆转录 聚合酶链式反应 (RT PCR)和Westernblot方法检测胱氨酸蛋白酶caspase 3的基因和蛋白表达及活化。结果　硫芥可导致淋巴细胞DNA损伤 ,Caspase 3在基因水平和蛋白水平上表达增加 ,同时发生活化作用 ,具有时间依赖效应。结论　硫芥通过caspase依赖途径诱导淋巴细胞毒作用 ,Caspase 3的表达与活化参与该过程 。Objective To investigate the manner and molecular mechanism of lymphocyte cytotoxic action induced by sulfur mustard. Methods Lymphocytes were separated from the spleen of Wistar rats and were cultured. DNA strand breaks were detected by fluorospectrophotometry. RT PCR and Western blotting were employed to detect the gene and protein expressions of caspase 3 and its activation. Results Sulfur mustard induced DNA strand breaks in the lymphocytes. Gene and protein expressions of caspase 3 were increased in a time dependent manner. Conclusion Sulfur mustard induces lymphocyte cytotoxic action by means of depending on the expression and activation of caspase 3.

关 键 词：硫芥 淋巴细胞 胱氨酸蛋白酶-3 凋亡 

分 类 号：R392.12[医药卫生—免疫学] R394-33[医药卫生—基础医学]