肺炎克雷伯菌TEM-1编码基因的克隆测序及基因定位  

Cloning, sequencing and positioning of the encoding gene of TEM-1 β-lactamase in Klebsiela pneumoniae

作　　者：朱卫民[1] 陈勇川[2] 钱元恕[3] 王宇明[1] 

机构地区：[1]第三军医大学附属西南医院全军感染病研究所,重庆400038 [2]第三军医大学附属西南医院药剂科,重庆400038 [3]汕头大学医学院药理学教研室,汕头515031

出　　处：《第三军医大学学报》2003年第13期1149-1152,共4页Journal of Third Military Medical University

摘　　要：目的　以我院临床分离的一株肺炎克雷伯菌 99 799株为研究对象 ,对其所产 β 内酰胺酶编码基因的序列、亚型及基因定位进行研究。方法　采用聚合酶链反应 (PCR) ,用 β 内酰胺酶通用引物、blaTEM 扩增获得 β 内酰胺酶编码基因的片段 ,克隆入pUCm T载体 ,以双脱氧链终止法测定核苷酸序列 ,再通过GenBank进行同源性检测确定其亚型和基因所在位置。结果　肺炎克雷伯菌 99 799株所含 β 内酰胺酶基因型为TEM型 ,与TEM 1编码基因序列完全相同 ,且同时位于细菌 15 0bp和 80bp的大、小质粒上。结论　重庆地区临床分离肺炎克雷伯菌有TEM 1βObjective To analyse the encoding gene of β lactamase of the clinically isolated Klebsiela pneumoniae 99 799 and to identify its subtype. Methods The gene of β lactamase derived from a stain of Klebsiela pneumoniae 99 799 named as was amplified by PCR. The purified PCR product was cloned into pUCm T vector and sequenced by Sanger's dideoxy chain termination composition method. Results The encoded gene of the bacterium was identified as TEM by PCR. It had the same sequence as the gene encoding TEM 1 and positioned at the 150 bp and 80 bp plasmids. Conclusion The TEM 1 β lactamase exists in Chongqing area.

关 键 词：Β-内酰胺酶 聚合酶链反应 序列分析 

分 类 号：R378.996[医药卫生—病原生物学] R394.33[医药卫生—基础医学]