我国四个自然保护区森林土壤中苏云金芽孢杆菌的分布  

Distribution of Bacillus thuringiensis in Forest Soil of Four Nature Reserves in China

作　　者：戴莲韵[1] 王学聘[1] 杨光滢[1] 张万儒[1] 

机构地区：[1]中国林业科学研究院林业研究所

出　　处：《林业科学研究》1993年第6期621-626,共6页Forest Research

基　　金：国家自然科学基金"我国森林土壤中苏云金芽孢杆菌生态分布的研究"

摘　　要：从我国四个不同立地带——长白山、百花山、鼎湖山、尖峰岭自然保护区,采集到林下0～5cm土层土壤样品75个,分离到苏云金芽孢杆菌39株。其中芽孢杆菌的总数量和苏云金芽孢杆菌的出土率和分离率有所差异,pH偏中性和含水量稍高的土壤,菌数较高;而与所测的各种土壤养分无明显相关。对39株苏云金芽孢杆菌的鉴定:17株属库尔斯塔亚种,6株属松蠋亚种,16株未定名。毒力测定表明:对杨扇舟蛾和马尾松毛虫幼虫的致死率在50％以上的菌株约占70％;对榆蓝叶甲和黄粉甲幼虫均无明显杀虫活性。A total of 75 forest soil samples at 0~5cm depth collected from four nature reserves (Chang Bai Shan, Bai Hua Shan, Deng Hu Shan, Jiang Feng Ling) in China were examined for account of Bacillus and Bacillus thu-ringiensis, for description of the distribution of B. thuringiensis and calculation of the rate of B. thuringiensis to Bacillus in these forest soil.39 isolates of B. thuringiensis obtained from the forest soil were identified by means of the characterization of physiology-biochemistry and esterase type. It indicated that among the 39 isolates,17 belong to B.t.subsp.kurstaki (H-serotype 3a3b), 6 belong to B.t.subsp.dendrolimus (H-serotype 4a4b),and 16 were different from known subspecies.These isolates were toxin-tested to insects. 70% of the 39 isolates were Lepidopteran-toxin:the mortality against Clostera anachoreta and Dendrolimus punctatus were above 50%. Coleoptero-toxin were not observed.

关 键 词：自然保护区 土壤 苏云金 芽孢杆菌 

分 类 号：S714.3[农业科学—林学]