乙型肝炎病毒S区和C区基因长片段扩增新方法的探讨  

A new long-fragment PCR for preC/C and preS/S of hepatitis B virus genomes

作　　者：苏海霞[1] 闫永平[1] 徐德忠[1] 李端[1] 卢娟[1] 

机构地区：[1]第四军医大学预防医学系流行病学教研室,陕西西安710033

出　　处：《第四军医大学学报》2003年第15期1417-1419,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 (39970 652 )

摘　　要：目的 :为HBV基因研究提供一个灵敏度和扩增效率均较佳的长片段PCR新方法 .方法 :应用自行设计的引物扩增血清中HBVS区和C区的 2 .5kb基因 ,并与HBV全长扩增方法进行比较 .结果 :HBV 2 .5kbPCR方法在HBsAg阳性患者血清中的阳性检出率明显高于全长PCR方法 (P <0 .0 5 ) ,相同反应条件下 2 .5kbPCR产物的平均A值和灰度总值也高于 3.2kb的全长基因组 (P <0 .0 5 ) .结论 :HBV2 .5kbPCR方法的灵敏度和扩增效率高于全长PCR方法 。AIM: To develop an efficient and sensitive long fragment polymerase chain reaction (PCR) for the study of hepatitis B virus (HBV). METHODS: 2.5 kb HBV genomes were amplified by PCR with new primers from serum and the result was compared with that of the full length PCR. RESULTS: The detection rate of 2.5 kb PCR was much higher than that of full length PCR from serum of HBsAg positive patients ( P <0.05). The average A value and gray level volume of the products of 2.5 kb PCR were also higher than those of the full length PCR. CONCLUSION: 2.5 kb PCR is more efficient and sensitive than full length PCR and can be used in the study the HBV correlative diseases.

关 键 词：肝炎病毒 乙型 基因组 PCR 

分 类 号：R182[医药卫生—流行病学]