含益髓灵兔血清诱导HL-60细胞凋亡与对Bcl-2抑制研究  

Study on Rabbit's Serum with Yi Sui Ling Inducing the Apoptosis of HL60 Cells and Inhibiting the Expression of Bcl-2 Gene

作　　者：高顺利[1] 王昕[1] 陈信义[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《中国中医基础医学杂志》2000年第8期27-29,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的 :探讨中药复方益髓灵治疗白血病作用机理 :方法 :在已知益髓灵具有诱导 HL- 60细胞凋亡的基础上 ,通过血清药理学方法 ,选择含不同浓度益髓灵初提物的兔药血清 ,与人急性早幼粒白血病 HL- 60细胞共同孵育不同时间后 ,观察兔药血清对 HL- 60细胞凋亡的影响 ,并对其发生凋亡机制进行初步探讨。结果 :益髓灵兔药血清有较强的诱导 HL- 60细胞凋亡作用 ,以 1 0 %兔药血清作用 48h效果最好 ,并发现其诱导凋亡机制与抑制 Bcl- 2基因表达相关。结论 :益髓灵进入动物体内也有诱导白血病细胞凋亡作用 ,其诱导白血病细胞凋亡是治疗白血病的关键机制之一。Objective:to study the mechanism of the compound preparation Yi Sui Ling to cure leukemia.Method:based on the fact that Yi Sui Ling can induce the apoptosis of HL60,by serologic pharmacological test,the author choosed rabbit serum samples with various density of Yi Sui Ling,cultured them with HL60 cells during different time,observed the effect of rabbit'serum with Yi Sui Ling on the apoptosis of HL60,and explored primarily the mechanism of its apoptosis.Results:Yi Sui Ling has a marked effect of inducing the apoptosis of HL60 cells.The best effect was produced by rabbit'serum with 10 percent concentration of Yi Sui Ling during 48 hours.The mechanism of its inducing apoptosis is related to its inhibiting the expression of Bcl-2 gene.Conclusion:Yi Sui Ling can induce the apoptosis of leukemia.cells even in animal's body,which is the key mechanism of Yi Sui Ling curing leukemia.

关 键 词：益髓灵 HL-60细胞 细胞凋亡 血清药理学 BCL-2基因 

分 类 号：R289.9[医药卫生—方剂学]