PURIFICATION OF L-METHIONINE AND N-ACETYL-D-METHIONINE FROM THE MIXTURE OF ENZYMATICALLY DEACYLATED N-ACETYL-DL-METHIONINE  

作　　者：YANXiaomin ZHAOLin SHAOJianhui TANXin SONGZhengxiao 

机构地区：[1]School of Chemical Engineering and Technology,Tianjin University Tianjin 300072,China [2]Slichem Liquid Material Co.Ltd.,Shijiazhuang,050061,China

出　　处：《Chinese Journal of Reactive Polymers》2004年第1期13-21,共9页中国反应性高分子（英文版）

基　　金：Tianjin Fund of Natural Science (043802611)

摘　　要：N-acetyl-D-methionine, NaAc and the remains of N-acetyl-L-methionine dramatically affect the purification of L-methionine when purified from the mixture of enzymatically deacylated N-acetyl-DL-methionine, leading to a low yield conventionally. Here, this paper reports a successful separation and purification of both L-methionine and N-acetyl-D-methionine by an H ion-exchange column. The pH, L-Met concentration and the ratio between the content of sodium cation and the ion-exchange capacity were optimized to obtain the maximum yield. Experimental results indicate that, under the optimized conditions, the yields of L-methionine and N-acetyl-D-methionine can reach as high as 85% and 75%, respectively.N-acetyl-D-methionine, NaAc and the remains of N-acetyl-L-methionine dramatically affect the purification of L-methionine when purified from the mixture of enzymatically deacylated N-acetyl-DL-methionine, leading to a low yield conventionally. Here, this paper reports a successful separation and purification of both L-methionine and N-acetyl-D-methionine by an H ion-exchange column. The pH, L-Met concentration and the ratio between the content of sodium cation and the ion-exchange capacity were optimized to obtain the maximum yield. Experimental results indicate that, under the optimized conditions, the yields of L-methionine and N-acetyl-D-methionine can reach as high as 85% and 75%, respectively.

关 键 词：Enzymatic deacylation hydrolysed of N-acetyl-DL-methionine Strong acid ion-exchanger separation of L-methionine and N-acetyl-D-methionine. 

分 类 号：O629.71[理学—有机化学]