应用微量淋巴细胞毒与流式细胞仪检测HLA-B27的比较  

Comparison of Microlymphocytotoxicity Test and Flow Cytometry in HLA-B27 Typing

作　　者：方勇飞[1] 钟兵[1] 李维[2] 王勇[1] 

机构地区：[1]重庆第三军医大学西南医院风湿科,400038 [2]重庆市血液中心,400015

出　　处：《中国误诊学杂志》2004年第8期1185-1187,共3页Chinese Journal of Misdiagnostics

摘　　要：目的 :探讨不同 HL A- B2 7检测方法之间的差异及各自的优缺点 ,为临床检验提供参考。方法 :分别采用微量淋巴细胞毒法及流式细胞仪法检测 134例血标本的 HL A- B2 7抗原 ,将两者结果进行比较分析。结果 :两种检测方法结果符合率为 94 .8%。微量淋巴细胞毒法检测强直性脊柱炎者 HL A- B2 7阳性率为 89.5 % ,对照组阳性率 11.0 % ,符合国内外大样本调查结果。结论 :两种检测方法均准确可靠 ,适合于有条件的医院开展。Objective:To discuss the difference and characteristics of two HLA-B27 testing methods,which can offer references for clinical testing. Methods: Microlymphocytotoxicity Test and Flow Cytometry were adopted to test HLA-B27 antigen in 134 samples and the following results were compared and analysed.Results:The accordance rate is 94.8% in two methods. B27-positive samples by Microlymphocyto-toxicity Test is 89.5% in patients with ankylosing spondylitis(AS) and 11.0% in control,which is accorded with the results of large-sample investigation.Conclusion:The two methods are both accurate and stable,which are adapted to the hospital with the instruments.

关 键 词：HLA-B27 抗原/分析 细胞毒性试验 免疫 流式细胞术 脊柱炎 强直性 

分 类 号：R593.33[医药卫生—内科学]